多肽抗原检测淋球菌抗体ELISA法的建立及应用  

Development of Enzyme Immunoassay of Gonorrhea Antibody with Peptide Antigen

作　　者：金怡[1] 俞炳耀[1] 甄子刚[1] 盛婪[1] 周文达[1] 

机构地区：[1]上海第二医科大学上海市医学检验重点实验室,上海200025

出　　处：《上海交通大学学报（医学版）》2001年第6期515-516,529,共3页Journal of Shanghai Jiao tong University:Medical Science

摘　　要：目的建立多肽抗原检测淋球菌抗体的ELISA方法。　方法用固相合成肽方法合成 2个淋球菌多肽抗原 ,建立测定淋球菌抗体的酶免疫测定 ,并对 89份正常人血清和 47份淋病患者血清进行检测。　结果批内测定变异系数为 7 1% ;批间测定变异系数为 10 7%。与金标记的试剂盒平行检测临床标本符合性良好。　结论该方法操作简单 ,试剂稳定 ,已制备成试剂盒在国内应用 。Objective To develope a sensitive method for the detection of gonorrhea antibody. Methods By compounding two gonorrhea peptide antigens, the ELISA method was established. The seram of 89 healthy persons and 47 patients with gonorrhe were examined by both ELISA method and immunogold filtration assay. Results The positive rate by the ELISA method was a little higher than that of immunogold filtration assay. The coefficient of variation intra-batch was 7 1% and the inter batch was 10 7%. Conclusion This method of enzyme immunoassay of gonorrhea antibody with peptide antigen is quite simple and the reagents are rather stable.

关 键 词：淋球菌 ELISA 多肽抗原 

分 类 号：R446.6[医药卫生—诊断学]