Mifepristone regulates expression of apoptosis related genes Fas and FasL in mouse endometrium  

米非司酮调控凋亡相关基因Fas和FasL在小鼠子宫内膜中的表达

作　　者：高飞[1] 许复华[1] 周新昌[1] 韩小彬 刘以训[1] 

机构地区：[1]中国科学院动物研究所生殖生物学国家重点实验室,北京中国100080

出　　处：《Acta Pharmacologica Sinica》2001年第6期48-53,共6页中国药理学报（英文版）

基　　金：WHO/Rockefeller Fundation (RF96020#78);"973"Program

摘　　要：AIM: To investigate the anti-implantation mechanism of mifepriston. METHODS: In situ hybridization and immunohistochemistry were applied to determine mRNA and protein. RESULTS: After mifepriston injection, the number of implantation sites were obviously reduced, mifepriston could inhibit the embryo implantation in mouse. The expression of apoptosis related genes, Fas and FasL, in mouse endometrium was also decreased after mifepriston treatment. CONCLUSION: The expression of apoptosis related genes Fas and FasL is regulated by mifepriston and the inhibitory effect of mifepriston on the embryo implantation may be mediated by action on the Fas/FasL system.目的:研究米非司酮抗着床机理。方法:用原位杂交法检测mRNA的表达,用免疫组化法定位蛋白质。结果:注射米非司酮后,胚胎的着床数明显下降,另外,注射米非司酮后与凋亡相关的基因Fas和FasL的表达也受到抑制。结论:米非司酮调控凋亡相关基因Fas和FasL的表达,且其抗胚胎着床作用可能是由Fas/FasL介导的。

关 键 词：MIFEPRISTONE APOPTOSIS in situ hybri-dization IMMUNOHISTOCHEMISTRY 

分 类 号：R96[医药卫生—药理学]