Vascular endothelial growth factor mRNA and its protein expression in decidua after terminating early pregnancy by mifepristone plus misoprostol  

米非司酮抗早孕出血副反应机理研究——蜕膜血管内皮细胞生长因子mRNA及蛋白表达观察(英文)

作　　者：黄丽丽[1] 石一复[1] 

机构地区：[1]浙江大学医学院附属妇产科医院,杭州310006

出　　处：《Chinese Medical Journal》2001年第5期69-71,109,共4页中华医学杂志（英文版）

摘　　要：Objective To investigate the action of mifepristone plus misoprostol on decidua at the level of vascular endothelial growth factor (VEGF) mRNA and its protein expression, as well as the mechanism of prolonged uterine hemorrhage after terminating early pregnancy with these drugs.Methods Forty-five decidua specimens were obtained from 45 pregnant women with amenorrhea of 6 - 7 weeks' duration, in which 15 women were treated with mifepristone and 15 were given mifepristone plus misoprostol. Enzyme-linked immunosorbant assay (ELISA) and reverse transcription-polymerase chain reaction (RT-PCR) were used to detect VEGF protein and mRNA levels in decidua.Results In all three groups, only the VEGF121 amplification product of 452 bp was visualized. The mRNA and protein levels of VEGF showed no significant differences among the three groups ( P ＞ 0.05).Conclusion In humans, early decidua VEGF121 mRNA is the main isoform. The action of mifepristone plus misoprostol on blood vessels in human decidua may be medicated by some factors other than VEGF.目的　研究米非司酮配伍米索前列醇药物流产后蜕膜血管内皮细胞生长因子 (VEGF)变化 ,探讨药物流产后子宫出血时间长的机理。方法　应用逆转录 聚合酶联反应 (RT PCR)技术及酶联免疫吸附试验 (ELISA)法观察正常早孕蜕膜组、米非司酮组、米非司酮米索药物流产组蜕膜VEGFmRNA及蛋白表达水平。结果　三组均仅见VEGF12 1mRNA表达 ;VEGF12 1mRNA和蛋白表达水平 3组间差异无显著性 (P >0 0 5)。结论　人早孕蜕膜主要表达VEGF12 1mRNA ;

关 键 词：mifepristone  ·   vascular endothelial growth factor   ·    decidua   ·  uterine hemorrhage 

分 类 号：R169.42[医药卫生—公共卫生与预防医学]