Effects of prolactin on HLA-DR and CD40 expressions by human thyrocytes  

催乳素对人甲状腺细胞表面HLA-DR和CD4 0表达的影响(英文)

作　　者：李静[1] 滕卫平[1] 单忠艳[1] 

机构地区：[1]中国医科大学第一临床学院内分泌科,沈阳110001

出　　处：《Chinese Medical Journal》2001年第11期31-36,104,共7页中华医学杂志（英文版）

基　　金：ThisworkwassupportedbytheNaturalScienceFoundationofLiaoningProvince (No 962 2 91)

摘　　要：Objective To study the effects of prolactin(PRL) on HLA-DR and CD40 expressions by human thyrocytes,and to investigate the possible mechanisms for PRL to affect the development of Graves'disease(GD).Methods Thyrocytes in secondary culture,which were from GD thyroid glands the tissues adjacent to the lwesions of multinodular goiter and andenoma(control group),were treated respectively with ovine PRL (oPRL),interferon-γ(IFN-γ),interlukin-4(IL-4)and oPRL plus IFN-γ(10U/ml)or IL-4(5ng/ml)for 7days.HLA-DR and CD40 expressions on the thyrocytes were determined by immunofluorescent staining and flow cytometry. Results oPRL(12.5ng/ml-1000ng/ml)had no significant direct effect on HLA-DR or CD40 expression.It did not significantly affect the stimulation of HLA-DR expressions on the rwo groups of thyrocyte treated with IFN-γ or on GD thyrocytes treated with IL-4.oPRL could antagonize the stimulation of CD40 expressions by IFN-γ and the inhibition by IL-4 on both groups of thyrocytes.The antagonizing effects were related to the concentration of PRL.IFN-γ-stimulated percentages of CD40+ thyrocytes and delta mean fluorescence intensity(dMF)in both thyrocyte sources were significantly reduced in the presence of 200ng/ml oPRL(both GD and Control:P<0.01and P<0.05,respectively;Control:P<0.05)and 1000ng/ml oPRL(GD:P<0.01;Control:P<0.05).oPRL caused significant increasing in IL-4-inhibited percentages of CD40+ cells from the two groups of thyrocytes at 12.5ng/ml and 1000ng/ml and dMF from GD thyrocytes at 1000ng/ml(P<0.05).Conclusions PRL can exert indirect effects on CD40 expressions on thyrocytes by antagonizing the modulatory actions of IFN-γand IL-4 with dose-related effects.This may be one important mechanism for PRL to affect the development of GD.目的　研究催乳素 (PRL)对人甲状腺细胞表面HLA DR和CD4 0表达的调节作用 ,以探讨PRL影响Graves’病(GD)发生、发展的可能机制。方法　将传代培养的GD和对照组 (取自结节性甲状腺肿和甲状腺腺瘤病变周围组织 )甲状腺细胞分别经羊PRL(oPRL)、IFN γ或IL 4以及oPRL和IFN γ(10U/ml)或IL 4 (5ng/ml)作用 7天。利用免疫荧光染色和流式细胞仪测定其表面HLA DR和CD4 0的表达。结果　oPRL(12 5 - 10 0 0ng/ml)对甲状腺细胞表面HLA DR和CD4 0的表达均没有明显的直接影响。而且它对IFN γ刺激两组甲状腺细胞及IL 4刺激GD甲状腺细胞表达HLA DR的作用亦无明显影响。oPRL能够拮抗IFN γ对两组甲状腺细胞表面CD4 0表达的刺激作用和IL 4的抑制作用。此拮抗作用与PRL的浓度有关。在 2 0 0 (GD :P <0 0 1及P <0 0 5 ;对照组 :P <0 0 5 )和 10 0 0ng/ml(P <0 0 1及P <0 0 5 )时 ,它能使IFN γ刺激的阳性细胞百分率及平均荧光强度 (dMF)均显著减少。在 12 5和 10 0 0ng/ml时能使受到IL 4抑制作用的两组甲状腺细胞中CD4 0 +细胞百分率以及在 10 0 0ng/ml时能使GD甲状腺细胞相应的dMF明显增加 (P <0 0 5 )。对于后者 ,与IL 4单独作用时比 ,oPRL在 12 5和 10 0 0ng/ml时GD甲状腺细胞的dMF明显增加 (P <0 0 5 )。结?

关 键 词：prolactin ·Graves' disease  ·  thyrocyte  · HLA-DR  ·  CD40 

分 类 号：R580.2[医药卫生—内分泌]