Role of β-adrenoceptor at different stages of bronchial asthma  

β肾上腺素能受体在支气管哮喘发病中的作用(英文)

作　　者：高海鹏[1] 薛全福[2] 林友华[1] 王立荣[2] 朱广瑾[2] 赵琪平[2] 陈育智[3] 

机构地区：[1]中日友好医院呼吸内科,北京100029 [2]中国医学科学院基础医学研究所病理生理研究室,北京100730 [3]首都儿科研究所,北京100020

出　　处：《Chinese Medical Journal》2001年第12期85-87,110,共4页中华医学杂志（英文版）

基　　金：theNationalNatureScienceFoundationofChina (No 395 70370 )

摘　　要：Objective To investigate the changes of β-adrenergic receptor (βAR) in peripheral lymphocytes and β2AR mRNA levels at different stages of bronchial asthma. Methods β2AR density and β2AR mRNA level in peripheral lymphocytes, cAMP and cGMP levels in blood plasma were estimated by radioligand binding assay, radioimmunoassay and RT-PCR.Results (1) Maximum bound volume (Bmax) and equilibrium dissociation constant (Kd) of β2AR of lymphocyte in asthma patients at remission stage were markedly higher than that in normal subjects, while cAMP levels in blood plasma showed no difference. Bmax of β2AR and cAMP levels in asthma patients at acute exacerbation stage were significantly lower than that in normal subjects, and Kds between these two groups were not much different. (2) Expression of β2ARmRNA in peripheral lymphocytes of asthmatics at remission stage was not significantly different compared with that in normals. Conclusions Amount and function of βAR and β2ARmRNA levels are related to asthmatic conditions. Changes of βAR and β2ARmRNA in asthma might rather be a pathological change accompanied by the course of the disease than a primary defect.目的　观察支气管哮喘不同状态时外周淋巴细胞肾上腺素能受体 (βAR)及β2 ARmRNA水平的变化。方法　用放射配基结合受体分析法 ,放射免疫法 ,逆转录聚合酶链反应 (PT PCR)及原位杂交等方法测定哮喘患者不同状态下 β2 AR密度 ,β2 ARmRNA水平 ,血浆cAMP和cGMP水平。结果　缓解期哮喘患者外周淋巴细胞 βAR的密度较正常人明显升高 ,血浆cAMP水平无显著差异 ,而发作期哮喘患者βAR及血浆cAMP水平均较正常人明显降低 ;缓解期哮喘患者外周淋巴细胞β2 ARmRNA水平与正常人无显著差异。结论　βAR数量、功能及其信使核糖核酸水平与哮喘不同发病状态有关 ,其改变仅为哮喘病程中伴随出现的变化 ,不是其发病的原发因素。

关 键 词：bronchial asthma   ·  β-adrenocepter   ·  cAMP   ·   cGMP   ·  mRNA 

分 类 号：R562.25[医药卫生—呼吸系统]