Physical location of rice Gm-6,Pi-5(t) genes in O.officinalis with BAC-FISH  

作　　者：QIN Rui WEI Wenhui JIN Weiwei HE Guangcun NING Shunbin YU Shunwu SONG Yunchun 

机构地区：[1]Key Laboratory of MOE for Plant Developmental Biology,Wuhan University,Wuhan 430072,China [2]Key Laboratory of Genetic Improvement of Crop,Huazhong Agricultural University,Wuhan 430072,China

出　　处：《Chinese Science Bulletin》2001年第8期659-661,707,共4页

基　　金：the National Natural Science Foundation ofChina (Grant No. 39870423) and the Doctorate Vesting Point Foundation of the State Education Commission of China (Grant No. 207980112).

摘　　要：A fluorescence in situ hybridization (FISH) procedure was adopted to physically map two rice BAC clones 24E21 and 4F22 linked to Gm-6 and Pi-5(t) in O. offi-cinalis. FISH results showed that the two BAC clones were located at 4L. The percentage distance from the centromere to the hybridization sites was 72 ± 2.62 for 24E21 and 54± 5.43 for 4F22, the detection rates were 52.70% and 61.2%. The results obtained from the BAC and plasmid clones, RG214 and RZ565 of cultivated rice and O. officinalis were the same. This suggested that the markers, RG214 and RZ565 of cultivated rice and O. officinalis were in the same BAC clones. The homologous sequences of Gm-6 and Pi-5(t) in O. officinalis were positions that signals existed on the 4L. Many signals were observed when no Cot-1 DNA blocked. This also showed that repetitive sequences were some ho-molgous between cultivated rice and O. officinalis. The identification of chromosome 4 of O. officinalis is based on Jena et al. (1994). In our study, we discussedA fluorescencein situ hybridization (FISH) procedure was adopted to physically map two rice BAC clones 24E21 and 4F22 linked toGm-6 andPi-5(t) inO. officinalis. FISH results showed that the two BAC clones were located at 4L. The percentage distance from the centromere to the hybridization sites was 72±2.62 for 24E21 and 54±5.43 for 4F22, the detection rates were 52.70% and 61.2%. The results obtained from the BAC and plasmid clones, RG214 and RZ565 of cultivated rice andO. officinalis were the same. This suggested that the markers, RG214 and RZ565 of cultivated rice andO. officinalis were in the same BAC clones. The homologous sequences ofGm-6 andPi-5(t) inO. officinalis were positions that signals existed on the 4L. Many signals were observed when no Cot-1 DNA blocked. This also showed that repetitive sequences were some homolgous between cultivated rice andO. officinalis. The identification of chromosome 4 ofO. officinalis is based on Jena et al. (1994). In our study, we discussed the possibility of physical map inO. officinalis with rice BAC clones.

关 键 词：O. OFFICINALIS FISH PHYSICAL LOCATION BAC clones. 

分 类 号：S511[农业科学—作物学]