Relationship among Photosys-tem Ⅱ carbonic anhydrase,extrinsic polypeptides and manganese cluster  

Relationship among Photosys-tem II carbonic anhydrase, extrinsic polypeptides and manganese cluster

作　　者：DAI Xinbin YU Yong ZHANG Rongxian YU Xinjian HE Peiming XU Chunhe 

机构地区：[1]Nanjing Agricultural University,Agronomy Department,Nanjing 210095,China [2]Shanghai Institute of Plant Physiology,Chinese Academy of Sciences,Shanghai 200032,China

出　　处：《Chinese Science Bulletin》2001年第5期406-409,共4页

基　　金：State Key Basic Research Development Plan (Grant No. G1998010100) and the National Natural Science Foundation of China (Grant No. 39770156).

摘　　要：Effects of Photosystem Ⅱ (PS Ⅱ) extrinsic polypeptides of oxygen-evolving complex and manganese clusters on PS Ⅱ carbonic anhydrase (CA) were studied with spinach PS Ⅱ membranes. The result supported that membrane-bound CA is located in the donor side of PS Ⅱ. The extrinsic polypeptides played an important role of maintaining CA activity. After removing manganese clusters, oxygen evolution activity was inhibited, but PSⅡ-CA activity was unchanged. It was concluded that CA activity is independent of the presence of manganese clusters, and was not directly correlated with oxygen evolution activity.Effects of Photosystem Ⅱ (PS Ⅱ) extrinsic polypeptides of oxygen-evolving complex and manganese clusters on PS Ⅱ carbonic anhydrase (CA) were studied with spinach PS Ⅱ membranes. The result supported that membrane-bound CA is located in the donor side of PS Ⅱ. The extrinsic polypeptides played an important role of maintaining CA activity. After removing manganese clusters, oxygen evolution activity was inhibited, but PSⅡ-CA activity was unchanged. It was concluded that CA activity is independent of the presence of manganese clusters, and was not directly correlated with oxygen evolution activity.

关 键 词：carbonic ANHYDRASE PS II oxygen evolution complex EXTRINSIC POLYPEPTIDE MANGANESE cluster. 

分 类 号：Q945[生物学—植物学]