The photodamage process of pigments and proteins of PSI complexes from Spinacia Oleracea L.  

作　　者：WEI Jie YU Hui LI Liangbi KUANG Tingyun WANG Jushuo GONG Yandao ZHAO Nanming 

机构地区：[1]Laboratory of Photosynthesis Basic Research,Institute of Botany,Chinese Academy of Sciences,Beijing 100093,China [2]Department of Biological Science and Biotechnology,Tsinghua University,Beijing 100084 China

出　　处：《Chinese Science Bulletin》2001年第21期1812-1816,共5页

基　　金：This work was supported by the National Natural Science Foundation of China (Grant No. 39890390); the State Key Basic Research and Development Program (Grant No. G1998010100); and the Innovative Foundation of Laboratory of Photosynthesis Basic Research

摘　　要：Purified PSI complexes from Spinacia Oleracea L. were exposed to the strong light (PFD=2300μmol m-2s-1) for various period. Along with the illumination the photo-damage process of pigments and proteins of PSI complexes was investigated using absorption, fluorescence, circular dichroism (CD) spectroscopy and SDS-PAGE. It was found from the optical absorption spectra that the maximal ab-sorbance of PSI complexes decreased and maximal peaks blue-shifted during the illumination, and the forth derivative spectra demonstrated that the absorbance decreasing at red region mainly resulted from the aborbance decreasing of the long wavelength Chla, implying that the long-wavelength Chla was readily to be bleached. The CD signals contributed by LHCI decreased more rapidly than other CD signals con-tributed by Chla and Carotenoid, indicating that the LHCI was more sensitive to light than core complexes. It was observed by SDS-PAGE that some small polypeptides of PSI complexes were damaged earlier than reactionPurified PSI complexes fromSpinacia Oleracea L. were exposed to the strong light (PFD=2300 μmol m?2s?1) for various period. Along with the illumination the photo-damage process of pigments and proteins of PSI complexes was investigated using absorption, fluorescence, circular dichroism (CD) spectroscopy and SDS-PAGE. It was found from the optical absorption spectra that the maximal absorbance of PSI complexes decreased and maximal peaks blue-shifted during the illumination, and the forth derivative spectra demonstrated that the absorbance decreasing at red region mainly resulted from the aborbance decreasing of the long wavelength Chla, implying that the long-wavelength Chla was readily to be bleached. The CD signals contributed by LHCI decreased more rapidly than other CD signals contributed by Chla and Carotenoid, indicating that the LHCI was more sensitive to light than core complexes. It was observed by SDS-PAGE that some small polypeptides of PSI complexes were damaged earlier than reaction center proteins PsaA and PsaB. Lhca3, Lhca2 and PsaD were the early degraded proteins during illumination. In addition, it is also observed that the insoluble-cohesive-denatured proteins appeared after prolonged illumination.

关 键 词：PSI pigment-protein complex PHOTODAMAGE LHCI absorption SPECTRA CD spectra. 

分 类 号：Q632[生物学—生物物理学]