hB1F and HNF1 work syner-gistically in the regulation of the function of HBV ENⅡ  

hB1F and HNF1 work syner-gistically in the regulation of the function of HBV EN II

作　　者：Cai, YN Li, M Zhou, Q Kong, YY Wang, Y 

机构地区：[1]Chinese Acad Sci, Shanghai Inst Biochem, Shanghai 200031, Peoples R China

出　　处：《Chinese Science Bulletin》2001年第2期133-136,共4页

基　　金：the National Natural Science Foundation of China (Grant No. 39893320); Science and Technology Fund of Shanghai (Grant No. 98JC14023).

摘　　要：Human B1 binding factor (hB1F) is a novel transcription factor cloned in our laboratory, which has the capacity of binding the Bl region of HBV enhancer Ⅱ (EN Ⅱ) and stimulating its activity. In this report, the functional relationship between hBlF and other liver-enriched activators of EN Ⅱ was investigated. The results of CAT assay indicated that there was only additive effect between hB1F and HNF4 as well as HNF3β. On the contrary, the presence of both hBlF and HNF1 would result in significant functional synergism. Further GST pull down assay indicated that hBlF could interact with HNFl directly in vitro. This study provides the evidence that hB1F and HNF1 work syn-ergistically in the regulation of the activity of HBV EN Ⅱ.Human B1 binding factor (hB1F) is a novel transcription factor cloned in our laboratory, which has the capacity of binding the Bl region of HBV enhancer Ⅱ (EN Ⅱ) and stimulating its activity. In this report, the functional relationship between hBlF and other liver-enriched activators of EN Ⅱ was investigated. The results of CAT assay indicated that there was only additive effect between hB1F and HNF4 as well as HNF3 β. On the contrary, the presence of both hB1F and HNF1 would result in significant functional synergism. Further GST pull down assay indicated that hB1F could interact with HNF1 directly in vitro. This study provides the evidence that hBlF and HNF1 work syn-ergistically in the regulation of the activity of HBV EN Ⅱ.

关 键 词：HEPATITIS B virus (HBV) HB1F HNF1 GST PULL down synergism. 

分 类 号：R341[医药卫生—基础医学]