Qualitative, quantitative and structural analysis of non-coding regions of classical swine fever virus genome  

作　　者：XIAO Ming ZHU Zhizhan ZHANG Chuyu 

机构地区：[1]Institute of Virology,College of Life Sciences,Wuhan University,Wuhan 430072,China [2]Department of Physics,Wuhan University,Wuhan 430072,China

出　　处：《Chinese Science Bulletin》2001年第15期1251-1258,共8页

基　　金：This work was supported by the National Basic Research Developmental Project (Grant No. G1999011900).

摘　　要：Classical swine fever virus (CSFV) is the pathogen of the swine fever. Understanding of the replication and expression of its genome is the basis for research of the pathogenicity for CSFV and development of antiviral drug. The noncoding regions (NCRs) of CSFV are the main regulatory regions for replication and expression. Qualitative, quantitative and structural analysis of 3’ NCRs and 5’ NCRs was done in order to locate the regulatory region in the NCRs and to character the NCRs. The sites, conserved sequences and structural elements related to the initiation of replication and expression were extracted from 17 3’ NCRs and 56 5’ NCRs. Those cis-elements may be initial recognition sites for replication, binding sites for transcription factors of host cell and interacting sites for initiation of protein synthesis, based on which a mechanism for the replication and expression of CSFV was brought forth. This research offers the direction for further experiment and lays down a basis for the research onClassical swine fever virus (CSFV) is the pathogen of the swine fever. Understanding of the replication and expression of its genome is the basis for research of the pathogenicity for CSFV and development of antiviral drug. The noncoding regions (NCRs) of CSFV are the main regulatory regions for replication and expression. Qualitative, quantitative and structural analysis of 3′ NCRs and 5′ NCRs was done in order to locate the regulatory region in the NCRs and to character the NCRs. The sites, conserved sequences and structural elements related to the initiation of replication and expression were extracted from 17 3′ NCRs and 56 5′ NCRs. Those ciselements may be initial recognition sites for replication, binding sites for transcription factors of host cell and interacting sites for initiation of protein synthesis, based on which a mechanism for the replication and expression of CSFV was brought forth. This research offers the direction for further experiment and lays down a basis for the research on hepatitis C virus (HCV), other pestiviruses and plus-strand RNA viruses.

关 键 词：classical swine FEVER VIRUS noncoding regions RNA replication information content PESTIVIRUS plus-strand RNA virus. 

分 类 号：S858.28[农业科学—临床兽医学]