Enhancement of the immobilization and discrimination of DNA probe on a biosensor using gold nanoparticles  

作　　者：ZHAO Hongqiu LIN Lin TANG Ji’an DUAN Mingxing JIANG Long 

机构地区：[1]Laboratory of Colloid and Interface Science,Center for Molecular Science,Institute of Chemistry,Chinese Academy of Sciences,Beijing 100080,China [2]Department of Biological Sciences and Botechnology,Tsing Hua University,Beijing 100084,China

出　　处：《Chinese Science Bulletin》2001年第13期1074-1077,共4页

基　　金：The work was supported by the National Natural Science Foundation of China (Grant Nos. 39990570 and 29733110).

摘　　要：Nanoparticles have been widely utilized in biological research in recent years. 1,6-Hexanedithiol was used as the medium in this work to attach Au nanoparticles on thegold plates. We studied the immobilization and hybridization of DNA probes onto the resulted plates using the quartz crystal microbalance method. Approximately 3-5 times adsorption enhancement of HS-DNA on the plates was obtained under our experimental conditions. Using this DNA sensor, the sensitivity was promoted by approximately three times to 0.35μg/mL.Nanoparticles have been widely utilized in biological research in recent years. 1,6-Hexanedithiol was used as the medium in this work to attach Au nanoparticles on the gold plates. We studied the immobilization and hybridization of DNA probes onto the resulted plates using the quartz crystal microbalance method. Approximately 3–5 times adsorption enhancement of HS-DNA on the plates was obtained under our experimental conditions. Using this DNA sensor, the sensitivity was promoted by approximately three times to 0.35 ?g/mL.

关 键 词：Au nanoparticles DNA sensor QUARTZ CRYSTAL microbal- ance. 

分 类 号：Q503[生物学—生物化学]