Identification of integrin-like in guard cells of Vicia faba  

作　　者：ZHANG Aihua HUANG Rongfeng WANG Xuechen YUAN Ming 

机构地区：[1]College of Biological Sciences,China Agricultural University,Beijing100094,China [2]Biotechnology Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,China [3]Key Lab of Plant Physiology&Biochemistry,The Ministry of Agriculture,Beijing 100094,China

出　　处：《Chinese Science Bulletin》2001年第13期1100-1103,共4页

基　　金：This work was supported by the Major State Basic Research Program of China (Grant No. G1999011704); and the National Natural Science Foundation of China (Grant No. 39730050).

摘　　要：We addressed the existence and localization of integrin-like in guard cells of Vicia faba by using a probe of polyclonal antibody against the human integrin (αvβ3/β5)-Western blot results showed that three integrins-like of about 47.3, 43.7 and 41.1 ku were detected from the preparation of membrane fragments of purified guard cell protoplasts. Further research with immunofluorescent scanning microscopy indicated that those integrins-like were localized on plasma membrane of guard cells, most nearing the dorsal wall, which is consistent with the reception of signals from epidermal cells to guard cells. Thus our results indicate, for the first time, that integrins-like are present at guard cell plasma membrane of Vicia faba.We addressed the existence and localization of integrin-like in guard cells ofVicia faba by using a probe of polyclonal antibody against the human integrin (αvβ3/β5). Western blot results showed that three integrins-like of about 47.3, 43.7 and 41.1 ku were detected from the preparation of membrane fragments of purified guard cell protoplasts. Further research with immunofluorescent scanning microscopy indicated that those integrins-like were localized on plasma membrane of guard cells, most nearing the dorsal wall, which is consistent with the reception of signals from epidermal cells to guard cells. Thus our results indicate, for the first time, that integrins-like are present at guard cell plasma membrane ofVicia faba.

关 键 词：integrins-like GUARD cells VICIAFABA signal transduction. 

分 类 号：R392.1[医药卫生—免疫学]