Molecular tagging of a genic male-sterile gene in rice  

Molecular tagging of a genie male-sterile gene in rice

作　　者：CHEN Xuewei, LI Shigui, WANG Wenming, LI Hanyun, ZHOU Kaida & ZHU Lihuang1. Institute of Genetics, Chinese Academy of Science, Beijing 100101, China 2. Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China 

出　　处：《Chinese Science Bulletin》2001年第1期66-69,共4页

摘　　要：The sterility of Pingxiang male-sterile rice (Pins), possibly derided from a spontaneous mutation in Pingxiang fertile rice (Pmf), was previously reported to be controlled by a single dominant nuclear gene. It can be restored to fertility either by a dominant epistatic gene or by higher temperature treatment at the early stage of inflorescence development. In order to tag the genie male-sterile gene, Pms, Pmf and Ce 64, a cytoplasmic male-sterile restoring line without the epistatic gene for Pms, were used to construct mapping populations. Two segregation populations, "(Pms/Ce 64) F1s (sterile plant)//Pmf" F1 and "Pms//(Pmf/Ce 64) F1" F1, were simultaneously developed. Subsequently, the genie male- sterile gene was mapped between a simple sequence length polymorphism marker, RM228, and a restriction fragment length polymorphism marker, G2155, with distances of 14.9 and 2.6 cM, respectively. The tagged dominant genie male-sterile gene is temporarily designated Ms-p.The sterility of Pingxiang male-sterile rice (Pms), possibly derided from a spontaneous mutation in Pingxiang fertile rice (Pmf), was previously reported to be controlled by a single dominant nuclear gene. It can be restored to fertility either by a dominant epistatic gene or by higher temperature treatment at the early stage of inflorescence development. In order to tag the genie male-sterile gene, Pms, Pmf and Ce 64, a cytoplasmic male-sterile restoring line without the epistatic gene for Pms, were used to construct mapping populations. Two segregation populations, '(Pms/Ce 64) Fls (sterile plant)//Pmf' Fl and 'Pms//(Pmf/Ce 64) Fl' Fl, were simultaneously developed. Subsequently, the genie male- sterile gene was mapped between a simple sequence length polymorphism marker, RM228, and a restriction fragment length polymorphism marker, G2155, with distances of 14.9 and 2.6 cM, respectively. The tagged dominant genie male-sterile gene is temporarily designated Ms-p.

关 键 词：RICE genie MALE-STERILITY MOLECULAR mapping MOLECULAR marker GENE localization. 

分 类 号：S511[农业科学—作物学]