Construction and characterization of a bacterial artificial chromosome library of rice 5460F  

作　　者：Jianmin Fu Xueping Qu Chuanyou Li Jianhang Jia Demin Jin Qian Wang Rencui Yang Bin Wang 

机构地区：[1]Chinese Acad Sci, Inst Genet, Beijing 100101, Peoples R China [2]Fujian Agr Univ, Fuzhou 350002, Peoples R China

出　　处：《Chinese Science Bulletin》1999年第17期1587-1592,共6页

摘　　要：Thermo-sensitive genie male sterile (TGMS) rice has a number of desirable characteristics for hybrid rice production. Many studies have demonstrated that the sterility of TGMS rice is controlled by a single recessive gene. It has been mapped for the first time on chromosome 8 and named tms1. Several AFLP markers which tightly linked to the tms1 gene have been identified recently. In order to develop a detailed physical map of the tms1 gene-encompassing region and finally clone the tms1 gene, a bacterial artificial chromosome (BAC)Thermo-sensitive genie male sterile (TGMS) rice has a number of desirable characteristics for hybrid rice production. Many studies have demonstrated that the sterility of TGMS rice is controlled by a single recessive gene. It has been mapped for the first time on chromosome 8 and namedtms 1. Several AFLP markers which tightly linked to thetms 1 gene have been identified recently. In order to develop a detailed physical map of thetms1 gene-encompassing region and finally clone thetms1 gene, a bacterial artificial chromosome (BAC) library of rice 5460F (the fertile mutant line of TGMS rice 5460S) using a modified vector pECBAC1 has been constructed. The constructed 5460F BAC library consists of 16 896 clones with an average insert size of 119 kb, which represents about 4.7 times rice haploid genome equivalents. Neither chloroplast nor mitochondrial DNA was detected from the library. The library was screened with three single copy sequence amplified fragment length polymorphism (AFLP) markers which tightly linked totms1 gene as probes and eight positive clones were identified.

关 键 词：RICE THERMO-SENSITIVE GENIE male STERILE BACTERIAL artificial chromosome. 

分 类 号：S511[农业科学—作物学]