Establishment of a genetic transformation system for maize inbred P9-10  

作　　者：Fengyong Zhou Guoying Wang Youju Xie Hongzhi Cui Sandui Guo Jingruil Dai 

机构地区：[1]China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100094, Peoples R China [2]China Agr Univ, Coll Plant Sci & Technol, Beijing 100094, Peoples R China [3]Chinese Acad Agr Sci, Ctr Biotechnol, Beijing 100081, Peoples R China

出　　处：《Chinese Science Bulletin》1999年第7期624-627,共4页

摘　　要：It was found that the supplement of 10^-4 mol/L AgNO₃ in N6 medium enhanced the induction of type Ⅰembryogenic calli from immature embryos of maize inbred P9-10. After high-osmotic treatment, the induced calli was taken as transformation recipient to be bombarded with plasmid pMG6 carrying synthetic Bt gene by PDS-1000/He genegun. A total of 14 resistant calli were obtained after screening subsequently on the mediums with gradually increasing selective pressure. 10 plants were regenerated from the resistant calli on 3 different induction media,and 8 out of the 10 regenerated plants were confirmed to be integrated with Bt gene by PCR and Southern blotting analysis. Results of ELISA showed that the Bt-protein content in the leaves of the transgenic plants varied between 20—200 ng/g fresh weight.It was found that the supplement of 10?4 mol/L AgNO3, in N6 medium enhanced the induction of type I embryogenic calli from immature embryos of maize inbred P9-10. After high-osmotic treatment, the induced calli was taken as transformation recipient to be bombarded with plasmid pMG6 carrying synthetic Bt gene by PDS-1000/He genegun. A total of 14 resistant calli were obtained after screening subsequently on the mediums with gradually increasing selective pressure. 10 plants were regenerated from the resistant calli on 3 different induction media, and 8 out of the 10 regenerated plants were confirmed to be integrated with Bt gene by PCR and Southern blotting analysis. Results of ELSA showed that the Bt-protein content in the leaves of the transgenic plants varied between 20–200 ng/g fresh weight.

关 键 词：MAIZE INBRED GENETIC TRANSFORMATION BT gene. 

分 类 号：S513[农业科学—作物学]