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作 者:Zou, JJ Dong, W Yang, QK Cao, YP Chen, SY
机构地区:[1]Chinese Acad Sci, Inst Genet, Plant Biotechnol Lab, Beijing 100101, Peoples R China [2]NE Agr Univ, Soybean Res Inst, Harbin 150030, Peoples R China
出 处:《Chinese Science Bulletin》1999年第5期452-456,共5页
摘 要:Frog-eye leaf spot of soybean caused by Cercospora sojina Hara is a kind of worldwide disease. Resistance to race 7 of C. sojina was found to be due to a single dominant gene through analyzing resistant behavior of the cross NEAU91212 (susceptible to race 7)× NEAU9674 (resistant to all races). 3 RAPD markers linked to the resistant gene Rcsc 7 were identified using the BSA method. 2 fragments of OPS03<sub>620</sub> and OPS03<sub>580</sub> amplified with primer OPS03 co-dominantly segregated in the F<sub>2</sub> individuals. The genetic distance between OPS03<sub>620</sub> and the resistant gene is 8.7 cM. According to the co-dominant marker, the accuracy of predicting the homozygous and heterozygous resistant F<sub>2</sub> individuals were 100% and 97.6%, respectively.Frog-eye leaf spot of soybean caused by Cercospora sojina Hara is a kind of worldwide disease. Resistance to race 7 of C. sojina was found to be due to a single dominant gene through analyzing resistant behavior of the cross NEAU91212 (susceptible to race 7) x NEAU9674 (resistant to all races). 3 RAPD markers linked to the resistant gene Rcsc 7 were identified using the BSA method. 2 fragments of OPS03(620) and OPS03(580) amplified with primer OPS03 co-dominantly segregated in the F-2 individuals. The genetic distance between OPS03620 and the resistant gene is 8.7 cM. According to the cc-dominant marker, the accuracy of predicting the homozygous and heterozygous resistant F-2 individuals were 100% and 97.6%, respectively.
关 键 词:CERCOSPORA sojina Hara resistant gene molecular MARKERS co-dominance.
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