Development of a quantitative RT-PCR system for the specific detection of human heat shock mRNA expression  

作　　者：Yanlin Wang Yufei Shen 

机构地区：[1]Chinese Acad Med Sci, Peking Union Med Coll, State Key Lab Med Mol Biol, Beijing 100005, Peoples R China

出　　处：《Chinese Science Bulletin》1999年第22期2058-2062,共5页

摘　　要：Heat shock proteins (HSPs), as molecular chaperones, play an important role under physiological condition and in the course of many diseases. It would therefore be valuable to determine the expression of cellular hsp gene quantitatively. Using DNA recombinant technique and in vitro transcription system, a complex internal control RNA has been prepared. After opti-Heat shock proteins (HSPs), as molecular chaperones, play an important role under physiological condition and in the course of many diseases. It would therefore be valuable to determine the expression of cellularhsp gene quantitatively. Using DNA recombinant technique andin vitro transcription system, a complex internal control RNA has been prepared. After optimization of conditions a RT-PCR system for specific quantification of cellularhsp70, hsp90α andhsp90β mRNA has been developed for the first time.

关 键 词：HEAT shock GENE MRNA QUANTITATIVE RT-PCR. 

分 类 号：R446.9[医药卫生—诊断学]