大鼠脊髓不完全性损伤后前角运动神经元的酶细胞化学改变  

ENZYME CYTOCHEMICAL CHANGES OF ANTERIOR HORN CELLS FOLLOWING INCOMPLETE SPINAL CORD INJURY IN THE RAT

作　　者：朱悦[1,2] 包峰[1,2] 王星铎[1,2] 

机构地区：[1]中国医科大学第一临床学院骨科 [2]中国医科大学脑研究所神经解剖学研究室

出　　处：《中国组织化学与细胞化学杂志》1999年第2期57-60,共4页Chinese Journal of Histochemistry and Cytochemistry

基　　金：辽宁省科学技术委员会基金

摘　　要：以改良Ａｌｅｎ氏法造成Ｗｉｓｔａｒ大鼠不完全性脊髓损伤，采用神经学功能评分法评定大鼠运动功能，应用定量酶细胞化学方法观察脊髓前角运动神经元内乙酰胆碱酯酶（ＡＣｈＥ）和酸性磷酸酶（ＡｃＰ）活性变化。结果显示：１．脊髓损伤后大鼠运动功能障碍，随后逐渐恢复。２．前角运动神经元内ＡＣｈＥ活性减弱、ＡｃＰ活性增强；随后酶活性呈逐渐恢复，四周时ＡＣｈＥ活性基本恢复正常。结果说明：大鼠脊髓不完全性损伤后运动功能变化与前角运动神经元的功能状态具有较强的相关性；前角运动神经元在不完全性脊髓损伤运动功能恢复中起重要作用。Incomplete spinal cord injury in the Wistar rats was produced using the modified Allen method. Rat motor function was evaluated by means of neurologic scoring. The acetylcholinesterase (AChE) activity and acid phosphatase activity in anterior horn cells were observed by using the quantitative enzyme cytochemistry. The results showed: 1. There appeared rat motor functional deficits after injury, followed by gradual recovery. 2. AChE activity in anterior horn cells dcreased and AcP activity increased; enzyme activity then gradually recovered and AChE activity restored basically to normal level four weeks after injury. The results indicate there is a strong correlation between the rat motor function and the functional state of anterior horn cells. The anterior horn cells play an important role in the motor functional recovery following incomplete spinal cord injury.

关 键 词：脊髓损伤 乙酰胆碱酯酶 酸性磷酸酶 运动功能 

分 类 号：R651.2[医药卫生—外科学]