肿瘤坏死因子和酯多糖对培养脑微血管内皮细胞NOS的影响  

THE EFFECT OF TNF α AND LPS TO THE NOS EXPRESSION ON CULTURED CEREBRAL MICROVASCULAR ENDOTHELIAL CELLS

作　　者：许彦钢[1,2] 刘勇[1,2] 张伟[1,2] 杨军[1,2] 

机构地区：[1]陕西省人民医院神经外科 [2]西安医科大学神经生物学研究中心

出　　处：《中国组织化学与细胞化学杂志》1999年第2期61-64,共4页Chinese Journal of Histochemistry and Cytochemistry

基　　金：陕西省自然科学研究项目基金

摘　　要：一氧化氮合酶（ＮＯＳ）活性与一氧化氮（ＮＯ）合成及血管功能关系密切，为了研究脑血管内皮细胞ＮＯＳ表达及其调节作用，本文采用脑微血管内皮细胞培养技术和组织化学方法，观察了内皮细胞ＮＯＳ的表达。未加刺激物对照组的脑微血管内皮细胞染色淡，阳性反应物主要聚集在细胞核周围胞浆中。加入肿瘤坏死因子－α（ｔｕｍｏｒｎｅｃｒｏｓｉｓｆａｃｔｏｒ－α，ＴＮＦ－α）或酯多糖（ｌｉｐｏｐｏｌｙｓａｃｃｈａｒｉｄｅ，ＬＰＳ）５ｍｉｎ后，ＮＯＳ染色开始增加，１ｈ达顶峰。以后呈下降趋势。ＬＰＳ比ＴＮＦ－α作用时间较长。In order to explore the effect of TNF α and LPS to the nitric oxide synthase (NOS) expression on cultured cerebrovascular endothelial cells of rat, the cell culture technique and histochemistry method were used to study the expression of NOS. The staining of NOS on the enormal Endothelial cells was light, the positive products were around the nucleus of the cells. With the induction of TNF α or LPS, the NOS staining became heavy after 5 minutes. Stimulated by TNF α or LPS, the NOS expression reached the highest after 1 hour and went down afterwards. Compared with TNF α, the staining of NOS induced by LPS appeared heavier and lasted for a longer time.

关 键 词：NOS 内皮细胞 组织化学 大鼠 

分 类 号：R329-34[医药卫生—人体解剖和组织胚胎学]