尖锐湿疣患者皮损中白介素2基因表达的研究  

THE STUDY ON EXPRESSION OF INTERLEUKIN-2 GENE IN LESION CONDYLOMA ACUMINATUM

作　　者：涂亚庭[1] 刘志香[1] 黄长征[1] 刘厚君[1] 

机构地区：[1]同济医科大学附属协和医院皮肤科,武汉430022

出　　处：《中国组织化学与细胞化学杂志》1999年第4期414-417,共4页Chinese Journal of Histochemistry and Cytochemistry

摘　　要：为探讨Ｔｈ１ 反应如白介素２ （ＩＬ－２） 产生在尖锐湿疣（ＣＡ） 发病及消退中的作用。本实验采用逆转录——聚合酶链反应（ＲＴ－ＰＣＲ） 技术检测了ＣＡ患者皮损组织ＩＬ－２ 的基因表达， 实验分为三组： 正常对照组（ｎ＝ １０）， 单纯病例组（ｎ＝ １８） 及γ－ 干扰素（ＩＦＮ－γ） 诱导组（ｎ＝ ６）。结果发现正常皮肤组织未检测到ＩＬ－２ ｍ ＲＮＡ， 在１８ 例单纯病例的ＣＡ患者有２ 例皮损ＩＬ－２ ｍ ＲＮＡ阳性， ６ 例ＣＡ患者注射ＩＦＮ－γ前局部皮损未检测到ＩＬ－２ ｍ ＲＮＡ， 而在皮损内注射ＩＦＮ－γ７２ 小时后有４ 例阳性。研究结果提示了ＩＦＮ－γ可诱导Ｔｈ１ 应答， Ｔｈ１ 应答在抵抗人类乳头瘤病毒（ＨＰＶ） 感染及清除ＨＰＶ中可能起着重要作用， 为临床应用ＩＬ－２ 及ＩＦＮ－γ治疗ＣＡ提供了理论依据。To investigate the role of Th1 response such as production of interleukin 2 (IL 2) in the pathogenesis and regression of condyloma acuminatum (CA), we measured the expression of IL 2 mRNA of surgically removed tissues by using reverse transcription__polymerase chain reaction (RT PCR) technique. The experiment was divided into three groups: normal control group (n=10), simple case group (n=18) and interferon γ induced group (n=6). The results were as follows: The expression of IL 2 mRNA was not detected in normal controls, but was detected in two lesions of 18 patients of CA IL 2 mRNA. In addition, IL 2 mRNA was detectedin 4 of 6 patients treated with intralesional IFN γ after 72 hours, whose IL 2 mRNA was negative before treated with IFN γ. The findings suggest that IFN γ induced Th1 response which might play a significant role in resistance to infection of human papillomavirus (HPV) or clearance of HPV. Thus it offers theoretical basis with IL 2 or IFN γ to treat CA in clinic.\;

关 键 词：尖锐湿疣 人类乳头瘤病毒 白介素2 干扰素Γ 

分 类 号：R752.53[医药卫生—皮肤病学与性病学]