SDF-1／CXCR4对脐血AC133^＋细胞趋化功能的影响  

Effects of SDF-1/CXCR4 on the Chemotaxis of Cord Blood AC133^+ Cells

作　　者：马艳萍[1] 马兰[1] 赵邵懂[2] 杨薏蓉[1] 杨林花[1] 

机构地区：[1]山西医科大学第二医院血液科,山西太原030001 [2]南京市儿童医院新生儿科,江苏南京210000

出　　处：《中国实验血液学杂志》2009年第2期408-411,共4页Journal of Experimental Hematology

基　　金：山西省青年基金,项目编号20041048

摘　　要：本研究探讨SDF1/CXCR4系统在脐血AC133+细胞趋化中的作用。用跨膜迁移实验(Transwell实验)确定SDF-1最佳趋化浓度,采用双色直接免疫荧光标记法和流式细胞仪测定脐血AC133+细胞表面CXCR4表达水平,并评价SDF-1最佳趋化浓度条件下细胞迁移率。结果发现,随着SDF-1浓度的增加,新鲜脐血AC133+细胞迁移率升高,但SDF-1浓度达到150ng/ml时迁移率趋于平稳;当CXCR4阻断型抗体作用后,迁移率与未加SDF-1组无差异。重组人造血生长因子组合SCF、FL、TPO体外培养AC133+细胞时,在培养的早期趋化因子SDF-1受体CXCR4的表达升高,同时迁移率也升高,但随着培养时间的延长,CXCR4表达量渐渐降低,迁移率随之降低。结论:AC133+细胞趋化率与CXCR4表达量间存在相关性。The aim of this study was to explore the effects of the stromal cell-derived factor （ SDF-1 ） and chemokine receptors （CXCR4） on chemotaxis of cord blood AC133^＋ cells . The optimal SDF-1 concentration was determined in Transwell System. The cell migration was calculated from the number of cells passing through polycarbonate membrane with 8 μm pore. The expressions of CXCR4 in fresh and cultured cord blood AC133^＋ cells were analyzed by flow cytometry with two-color direct immunoflurescence. The results showed that the chemotactic rate of fresh cord blood AC133^＋ cells increased along with increasing concentrations of SDF-1, however, it tended to be stable when the concentration of SDF-1 reached 150 ng/ml. There was no difference in the chemotactic rate of cord blood AC133^＋ ceils between the group with SDF-1 adding CXCR4-blocking antibody and the group without SDF-1. When AC133^＋ cells were cultured in vitro with hemopoietic growth factors, the expression of CXCR4 increased at the early stage, but decreased gradually along with time extending. In conclusion, there was correlation between the chemotactic rate of AC133^＋ cells and the expression of chemokine receptor CXCR4.

关 键 词：脐血 AC133^+ 细胞 SCFl/CXCR4 SDF-1 CXCR4 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]