人端粒酶催化亚基hTERT基因启动子的克隆  

Cloning and characterization of human telomerase catalytic subunit (hTERT) gene promoter

作　　者：黄君健[1] 李杰之[1] 林坚[1] 蒋晓山[1] 高朝晖[1] 黄翠芬[1] 

机构地区：[1]军事医学科学院生物工程研究所,北京100850

出　　处：《生物技术通讯》1999年第3期167-170,共4页Letters in Biotechnology

基　　金：国家自然科学基金 (3 9870 783 )资助

摘　　要：为了确定人端粒酶催化亚基 h TERT基因的启动子结构特征 ,采用 Panhandle PCR技术 ,从正常人外周血单核细胞基因组 DNA中扩增 h TERT基因 5′端上游旁侧序列 ,结果获得了 h TERT基因翻译起始位点上游 2 0 90 bp的基因组 DNA序列。序列分析表明 h TERT基因的启动子区域缺少典型真核启动子的核心元件 ( TATA box和 CAAT box) ,但含有多个已知转录因子蛋白结合的核心序列 ,如 E box及 Sp1核心序列。提示 h TERT基因的表达可能受特殊的转录因子调控 ,这些转录因子的激活可能与癌变细胞中 hIn order to characterize the promoter of human telomerase catalytic subunit (hTERT) gene,the 5' flanking sequences of the hTERT gene were amplified from genomic DNA isolated from a healthe Chineseperipheral blood mononuclear cells by Panhandle PCR.A fragment of 2 181 bp genomic DNA upstream from the translational initiation site of the hTERT gene was cloned.The result of sequencing indicates that the hTERT gene promoter lacks of TATA box and CAAT box,but contains several known transcriptional factors binding consensus sequences such as E box and Spl and Apl elements.These findings suggest that the expression of the hTERT gene is controlled by specific transcriptional factors.The activation of these factors may be corresponded for the re-constitutive expression of the hTERT gene during cellular carcinogenesis.

关 键 词：端粒酶 HTERT基因 启动子 PanhandlePCR 

分 类 号：Q785[生物学—分子生物学]