红车轴草提取物对小鼠同种异基因皮片移植的影响  

Effects of Trifoliumpratense Leguminosae extract on mouse allogenetic skin transplantation

作　　者：杨志[1] 黄秀艳[1] 狄静芳[1] 曾耀英[1] 

机构地区：[1]暨南大学组织移植与免疫实验中心,广州510632

出　　处：《中华中医药杂志》2009年第4期443-446,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：973国家重大基础研究项目(No.2006CB504201;2004CB720100);广州市科技局科技攻关重点项目(No.2006Z-E0091);广东省基金项目(No.2006B36030016)

摘　　要：目的:探讨红车轴草提取物(Trifoliumpratense Leguminosaeextract,TLE)对小鼠同种异基因皮片移植的影响。方法:受体BALB/c小鼠分为生理盐水对照组(physiologicsaline,PS)与TLE处理组,通过背-背法进行C57BL/6的全厚皮皮片移植,以观察TLE处理组移植皮片生长情况是否优于PS组。取受体小鼠淋巴细胞进行羧基荧光素乙酰乙酸(carboxyfluorescein diacetate,succinimidyl ester,CFDA-SE)标记的增殖追踪及混合淋巴细胞反应。结果:尾静脉注射TLE25μg/kg每天的处理组移植皮片生长情况优于PS组。TLE处理组BALB/c小鼠淋巴细胞增殖低于PS组。结论:TLE可能参与了小鼠免疫系统调节,诱导了其对同种异基因皮片的耐受。Objective： To observe the effect of Trifoliumpratense Leguminosae extract （TLE） on mouse allogenetic skin transplantation. Methods： Recipient BALB/c was divided into physiologic saline （PS） group and TLE group, full-thickness skins were transplanted through back to back method from donor C57BL/6. The allogenetic transplanted skin growth condition was observed. The proliferation of lymphocytes of recipient mice in vitro were detected by CFDA-SE stain and mixed lymphocyte reaction respectively. Results： The allogenetic transplanted skin injected with TLE 25g/kg per day by vena caudalis growed better than that in PS group. The proliferation of lymphocyte in TLE group was smaller than that in PS group. Conclusion： TLE maybe participate in the regulation of mouse immune system and induce its tolerance to the allogenetic transplanted skin.

关 键 词：红车轴草提取物 皮片移植 同种异基因 增殖 混合淋巴细胞反应 实验研究 

分 类 号：R285.5[医药卫生—中药学]