膜片钳技术和酶法分离豚鼠初级精母细胞的比较  

Comparison of patch-clamp technique with enzymatic dissociation in isolating primary spermatocytes in guinea pigs

作　　者：李云阁[1] 李云义[1] 沙建慧[1] 刘焕珠[1] 沈楠[1] 战术[2] 姜丽萍[1] 

机构地区：[1]空军医学高等专科学校,吉林市132013 [2]白求恩医科大学,长春市130021

出　　处：《空军医高专学报》1999年第1期1-3,共3页

摘　　要：应用膜片钳技术与酶法分离细胞技术,分别获得豚鼠单个初级精母细胞,观察了光镜下不同浓度伴刀豆蛋白A、不同时间对精母细胞贴壁及经膜表面清洁后封接和破膜的影响.结果表明,膜片钳技术优于酶法分离,细胞贴壁采用0.125g/L伴刀豆蛋白A作用10min为宜,膜表面清洁处理以0.35g/L胶原酶作用5min易于封接和破膜.Single primary spermatocyles in guinea pigs were obtained both by patch-clamp technique and by enzymatic dissociation and observed under microscope for the effects of concanavalin A in different concentrations and for the influence of different time lengths on its setdement to the bottom of 35 mm Petri dishes.The result showed that patch-clamp technique is superior to enzymatic dissociation, the suitable concentration was 0.125 g/L and the suitable time length was 10 min; for cleaning the surface of membrane,0.35g/L pipan was suitable and 5 min was enough.

关 键 词：精母细胞 分离 膜片钳技术 

分 类 号：R33[医药卫生—人体生理学]