外源一氧化氮对NaCl胁迫下番茄幼苗活性氧代谢的影响  

Effects of exogenous nitric oxide on reactive oxygen metabolism in tomato seedlings under NaCl stress

作　　者：吴雪霞[1] 陈建林[1] 查丁石[1] 朱为民[1] 

机构地区：[1]上海市农业科学院园艺研究所,上海市设施园艺技术重点实验室,上海201106

出　　处：《植物营养与肥料学报》2009年第2期422-428,共7页Journal of Plant Nutrition and Fertilizers

基　　金：沪农科攻字(2006)第2-1号项目资助

摘　　要：研究了在100 mmol/L NaCl胁迫下,外源NO供体硝普钠(SNP)处理对耐盐性不同的2个番茄品种("沪番1480"和"沪番2496")幼苗叶片活性氧(ROS)水平和保护酶活性的影响。结果表明,外源NO提高了盐胁迫下SOD、POD、CAT和APX活性,AsA和GSH含量以及脯氨酸和可溶性糖含量,降低了MDA含量和O2产生速率;与耐盐品种"1480"相比,NO处理对盐敏感品种"2496"效果更明显。表明外源NO通过促进盐胁迫下保护酶活性、抗氧化剂和渗透调节物质的提高,降低ROS水平,缓解NaCl胁迫对番茄幼苗生长的抑制作用,增强植株的耐盐能力。Effects of NO sodium nitropprusside （SNP, an exogenous nitric oxide donor） on reactive oxygen species and protective enzyme activities were studied in the leaves of two tomato euhivars, ＂Hufan 1480＂ and ＂Hufan 2496＂, under 100 mmol/L NaCl stress. Results showed that nitric oxide not only increased the activities of SOD, POD, CAT and APX, but also increased the concentration of AsA and GSH, and of proline and soluble sugar in leaves. Results also showed decreased MDA concentrations and O2^- producing rate under NaCl treatments. The effect of NO treatment in salt- sensitive ＂Hufan 2496＂ was more effective than that in salt-tolerant ＂Hufan 1480＂ under salt stress. Results suggested that NO could increase protective enzymatic activities, contents of osmotic adjustment substances and antioxidants under NaCl stress. Thus, NO played an important role in enhancing the resistance to NaCl stress of tomato seedlings and elevated the salt tolerance of plants through keeping low ROS contents.

关 键 词：一氧化氮 NACL胁迫 番茄幼苗 活性氧 保护酶 

分 类 号：S641.2[农业科学—蔬菜学] Q945.78[农业科学—园艺学]