中药复方别敏胶囊对呼吸道变应性小鼠内分泌免疫功能的调节作用(英文)  

Effects of Chinese herbal formula Biminne in regulating endocrine-immune function in mice with airway allergy

作　　者：张素琴[1] 沈自尹[1] 胡国让[2] 刘闰红[1] 张新民[1] 

机构地区：[1]复旦大学华山医院中西医结合研究所,上海200040 [2]澳大利亚悉尼大学Concord医院免疫与变态反应科

出　　处：《中西医结合学报》2009年第4期349-353,共5页Journal of Chinese Integrative Medicine

基　　金：上海市科学技术委员会攻关项目(No.02DZ19128)

摘　　要：目的:观察中药复方别敏胶囊对呼吸道变应性模型小鼠内分泌免疫功能的影响。方法:利用卵清蛋白建立呼吸道变应性小鼠模型,采用苏木精和伊红染色观察肺组织病理变化,运用流式细胞仪检测脾淋巴细胞中Th1和Th2细胞变化,利用酶联免疫吸附测定方法检测脾淋巴细胞培养上清白细胞介素4(interleukin-4 ,IL-4)、白细胞介素5(interleukin-5 ,IL-5)、干扰素γ(interferon-gamma ,INF-γ)水平及血清皮质酮浓度。结果:别敏能够明显减轻模型小鼠肺组织的炎症反应,降低模型小鼠Th2细胞及其细胞因子IL-4、IL-5水平,升高Th1细胞及其细胞因子INF-γ水平,显著抑制模型小鼠内源性皮质酮水平的下降。结论:别敏对机体内分泌免疫系统功能紊乱的调节作用可能是其治疗呼吸道变应性疾病的重要机制之一。Objective： To investigate the effects of Biminne, a traditional Chinese herbal formula, in regulating the endocrine-immune function in mice with airway allergy. Methods： Forty BALB/c mice were randomly divided into four groups, control group, untreated group, Biminne group, and dexamethasone （Dex） group. The murine model of airway allergy was established by intraperitoneal injection sensitization and intranasal challenge with ovalbumin （OVA）. On day 28, the mice were sacrificed and the sera were collected. The lung tissue of mice was removed for haematoxylin-eosin staining and the pathological changes were observed. The spleen cells of mice were isolated and the ratio of Th2/Th1 was determined by flow cytometry. The isolated spleen cells were also cultured in 24-well plates in the presence of concanavalin A and the supernatants were collected after 72-hour culture. Levels of interleukin-4 （IL-4）, IL-5 and interferon-gamma （IFN-γ） in the supernatants and the corticosterone level in sera were analyzed by enzyme-linked immunosorbent assay. Results. Biminne significantly relieved the inflammatory reaction in the lung tissue of airway allergic mice. Compared with the control group, the Th2 cells in the untreated group increased, while the IFN-γ level decreased and the ratio of Th2/Th1 increased significantly, showing a Th2 response priority. Biminne treatment could down-regulate the Th2 cells and their cytokines IL-4 and IL-5, and up-regulate the Th1 cells and their cytokine IFN-γ. So the ratio of Th2/Th1 decreased significantly, shifting to the control level. In addition, Biminne also inhibited the reduction of the serum corticosterone of sensitized mice. Conclusion： The Chinese herbal formula Biminne can reverse the functional disorder of the endocrine-immune system, which may explain its therapeutic effects on airway allergic disease.

关 键 词：变应性 白细胞介素4 白细胞介素5 干扰素Γ 皮质酮 小鼠 

分 类 号：R285.5[医药卫生—中药学]