红藤颗粒剂对大鼠异位子宫内膜血管内皮生长因子及其受体胎肝激酶-1表达的影响  

Effects of Caulis Sargentodoxae Granule on expressions of vascular endothelial growth factor and its receptor-2 in rats with endometriosis

作　　者：曹阳[1] 张婷婷[2] 谢淑武[3] 朱焰[3] 束兰娣[1] 宋知理[1] 曹霖[3] 戴德英[1] 

机构地区：[1]上海中医药大学曙光医院妇科,上海200021 [2]上海中医药大学岳阳中西医结合医院妇科,上海200437 [3]上海市计划生育科学研究所生殖药理实验室,上海200032

出　　处：《中西医结合学报》2009年第4期360-365,共6页Journal of Chinese Integrative Medicine

基　　金：上海市重点学科(第三期)中医妇科学建设资助项目(No.S30303)

摘　　要：目的:观察红藤颗粒剂对子宫内膜异位症大鼠异位内膜的抑制作用以及对异位内膜血管内皮生长因子(vas-cular endothelial growth factor,VEGF)及其受体2胎肝激酶-1(fetalliverkinase-1,Flk-1)表达的影响。方法:采用自体移植法建立子宫内膜异位症大鼠模型,48只SD大鼠随机分为模型组、去势组、米非司酮(5mg/kg)组和低(20g/kg)、中(40g/kg)、高(80g/kg)剂量红藤颗粒剂组。连续灌胃给药21d后处死大鼠,用电子数显卡尺测定并计算异位内膜的体积,采用免疫组织化学法检测各组异位内膜VEGF及其受体Flk-1的表达。结果:红藤颗粒剂低剂量组异位内膜体积与模型组比较差异无统计学意义(P>0.05),其他组异位内膜体积均明显低于模型组,差异有统计学意义(P<0.05)。病理组织学观察发现,除红藤颗粒剂低剂量组外,其余各组异位内膜厚度明显低于模型组,差异有统计学意义(P<0.01),异位内膜呈退化趋势。免疫组织化学检测发现,VEGF主要表达于异位内膜腺上皮及血管内皮细胞胞浆,散在于周围基质细胞胞浆。除红藤颗粒剂低剂量组外,各治疗组异位内膜VEGF和Flk-1平均灰度值与模型组比较,差异有统计学意义(P<0.05,P<0.01)。结论:中药红藤颗粒剂可通过抑制异位内膜VEGF及其受体Flk-1的表达,阻止新血管的生成,抑制大鼠异位子宫内膜的生长并使其萎缩。Objective： To investigate the effects of Caulis Sargentodoxae Granule （CSG）, a compound traditional Chinese herbal medicine for treating endometriosis, on expressions of vascular endothelial growth factor （VEGF） and its receptor-2 fetal liver kinase-1 （FIk-1） in rats with endometriosis. Methods： A rat model of endometriosis was established by autotransplant of endometria. Forty-eight endometriosis rats were randomly divided into 6 groups： untreated group, castrate group, mifepristone group （5 mg/kg） and low- （20 g/kg）, medium- （40 g/kg） and high-dose （80 g/kg） CSG group. After 21-day consecutive treatment, the expressions of VEGF and its receptor Flk-1 in ectopic endometria were detected by immunohistochemical method, and the volume of the ectopic endometria was also measured by using electronic digital calipers. Results. Compared with the untreated group, the endometrial volumes in the treated groups were significantly decreased （P〈0. 05） except for the low-dose CSG group. Histopathologic observation showed that the epithelial layer of ectopic endometrium was less and the thickness of epithelial layer was thinner than those in the untreated group （P〈0. 01）, but the low-dose CSG group had few changes. The VEGF and its receptor Flk-1 expressed abundantly in the cytoplasm of the glandular epithelium and vascular endothelial cell in all the groups. Compared with the untreated group, the expressions of VEGF and Flk-1 of ectopic endometrium in the CSG-treated groups, the mifepristone group and the castrate group were significantly decreased （P〈0. 05, P〈0. 01）. Conclusion： CSG can treat the endometriosis in rats, and its action may be associated with decreasing the expressions of VEGF and its receptor Flk-1 in ectopic endometrium.

关 键 词：红藤颗粒剂 子宫内膜异位症 血管内皮生长因子 血管内皮生长因子受体2 大鼠 

分 类 号：R285.5[医药卫生—中药学]