复黄生肌愈创油膏对大鼠创面肉芽组织中Ⅰ型和Ⅲ型胶原表达的影响  

Effects of Fuhuang Shengji Yuchuang Ointment on expressions of types Ⅰ and Ⅲ collagens in granulation tissue of wound in rats with diabetes

作　　者：肖秀丽 王振宜[2] 唐汉钧[3] 刘晓鸫[3] 

机构地区：[1]上海市宝山区中医医院中医外科,上海201900 [2]上海中医药大学岳阳中西医结合医院肛肠科,上海200437 [3]上海中医药大学龙华医院中医外科,上海200032

出　　处：《中西医结合学报》2009年第4期366-371,共6页Journal of Chinese Integrative Medicine

基　　金：高等学校博士学科点专项科研基金资助项目(No.20050268012)

摘　　要：目的:动态观察复黄生肌愈创油膏(简称复黄膏)对Wistar大鼠糖尿病创面新生肉芽组织中Ⅰ型和Ⅲ型胶原表达的影响。方法:雄性Wistar大鼠54只,随机分为创面对照组、模型组和复黄膏组。制备糖尿病合并皮肤创面模型,复黄膏组给予复黄膏外敷,模型组给予生理盐水纱布外敷,1次/d。造模后分别于第3天和第11天分批处死动物,采用免疫组织化学技术观察不同时段创面新生肉芽组织中Ⅰ型和Ⅲ型胶原含量的变化。结果:在创面修复的第3天,3组间Ⅰ型胶原的表达差异无统计学意义;复黄膏组Ⅲ型胶原的表达高于模型组(P<0.05),与创面对照组比较,差异无统计学意义。在创面修复的第11天,复黄膏组Ⅰ型和Ⅲ型胶原的表达均高于模型组(P<0.05),但与创面对照组比较差异无统计学意义。结论:复黄膏通过促进难愈性创面组织中Ⅰ、Ⅲ型胶原增殖及调节二者的平衡,起到促进创面愈合的作用。Objective： To observe the effects of Fuhuang Shengji Yuchuang （FHSJYC） Ointment, a compound traditional Chinese herbal medicine, on the expressions of types Ⅰ and Ⅲ collagens in granulation tissue of wound in rats with diabetes. Methods： Fifty-four male Wistar rats were randomly divided into three groups, wound control group, normal saline （NS） group and FHSJYC Ointment group. Diabetes was induced by injection of 1.5% alloxan and oral gavage of 5% glucose, and skin wound was made in rats of the NS group and the FHSJYC Ointment group. Skin wounds of the rats in the FHSJYC Ointment group were treated with FHSJYC Ointment gauze dressing, while those in the NS group were treated with NS gauze dressing once daily. The rats were executed in turn on the third day and the eleventh day of the treatment, and the changes of the content of types Ⅰ and Ⅲ collagens in the wound granulation tissue were observed by immunohistochemical technology. Results： Compared with the NS group, the wound closure index in the FHSJYC Ointment group was increased （P〈0. 05）. After 3-day treatment, the expression of type Ⅰ collagen showed no significant differences among the three groups, while the expression of type Ⅲ collagen in the FHSJYC Ointment group was higher than that in the NSgroup （P〈0.05）, similar with that in the wound control group. After 11-day treatment, the expressions of both types Ⅰ and type Ⅲ collagens in the FHSJYC Ointment group were higher than those in the NS group （P〈0.05）, similar with those in the wound control group. Conclusion： FHSJYC Ointment can affect the process of wound healing by promoting and regulating the expressions of types Ⅰ and Ⅲ collagens.

关 键 词：复黄生肌愈创油膏 伤口愈合 胶原Ⅰ型 胶原Ⅲ型 大鼠 

分 类 号：R285[医药卫生—中药学]