棉枯萎镰刀菌血清学检测方法和技术的研究  

Studies on the Methods and Techniques for Serodetection of Fusarium oxysporum Schl.f.sp.vasinfectum(Atk.)Snyder et Hansen

作　　者：李启新 杜琦[1] 陈长法 李祥[2] 

机构地区：[1]青岛动植物检疫局,青岛266002 [2]华中农业大学植保系

出　　处：《中国农业大学学报》1998年第S1期47-53,共7页Journal of China Agricultural University

摘　　要：以棉枯萎镰刀菌公安菌株为材料,用物理法和化学法提取抗原并制备抗血清,并选有代表性的抗血清对其特异性和灵敏度分别采用4种检测方法进行了检测比较研究。结果表明:(1)采用 Richard’s培养基振荡培养菌株,化学法提取菌丝孢子抗原,按改进的 J.E.Devay 设计的免疫方案免疫,能产生效价高达1:64的抗血清。(2)琼扩、比较对流免疫电泳、间接 ELISA,SPA-ELISA 等4种方法检测其特异性具有鉴别到尖孢镰刀菌种的水平的特异性,但不能鉴别专化型和生理小种。并用上述方法检测抗血清灵敏度。The pathogen Fusarium oxysporum f.sp.vasinfectum'Gong-an'strain of cotton fusarium wilt was tested.The antigen protein was extracted by physical and chemical methods and antisera were produced with Japanese white rabbits.Two representative anti- sera As2 and As10 were used to study their specificity and sensitivity with four methods. The results indicated that:(1)The strains were cultured vibrantly in Richard's medium. Extracts from mycelia and conidia were treated with chemical method and immuned rabbits as described by J.E.Devay with modifications,which produced a high titre antiserum.(2) The speciality of antisera detected by agar dimension diffusion(ADD),comparative im- muno-osmopheresis(CIOP),indirect ELISA,and SPA-ELISA was at the species level of Fusarium oxysporum,but not at formae species or physiological races level.We also detect- ed the sensitivity of As with four methods mentioned above.

关 键 词：棉枯萎病 血清学 琼脂双扩散 对流免疫电泳 间接 ELISA SPA-ELISA 

分 类 号：S435.621[农业科学—农业昆虫与害虫防治]