微生物发酵液中布洛芬含量的反相高效液相色谱测定  

Determination of Ibuprofen in Microbial Enzyme Asymmetrical Hydrolysis Fermentation Liquid by Reversed-Phase High Performance Liquid Chromatography

作　　者：刘勇[1] 罗氚芸[2] 刘亚君[1] 古昆[3] 

机构地区：[1]云南大学省微生物发酵重点实验室,昆明650091 [2]云南师范大学农村能源重点实验室,昆明650092 [3]云南大学化学系,昆明650091

出　　处：《云南大学学报（自然科学版）》1998年第S3期443-444,共2页Journal of Yunnan University(Natural Sciences Edition)

基　　金：云南省应用基础研究基金

摘　　要：应用反相高效液相色谱法测定微生物不对称酶解发酵液中布洛芬的含量，结果表明含量测定准确，重现性好．A RP-HPLC method for quantitative determination of ibuprofen in microbial enzyme asymmetrical hydrolysis fermentation liquid was developed. Operating conditions are PE C18 column, 150 x 4. 6mm, methanol-water (80:20 volume ratio) eluent with 1. 5 ml/min and UV-225 nm detection. The results show the method simple rapid and acurate.

关 键 词：布洛芬 反相高效液相色谱 含量测定 

分 类 号：O657.7[理学—分析化学]