烧伤血清对大鼠心肌细胞L型钙通道电流的激活作用  

Postburn serum activated L-type calcium channel current in isolated cardiac myocytes of adult rats

作　　者：沈洪兴[1,2] 陈玉林[1,2] 方之扬[1,2] 梅岩艾[1,2] 黄洛秀[1,2] 葛绳德[1,2] 刘世康[1,2] 夏照帆[1,2] 

机构地区：[1]第二军医大学长海医院烧伤科 [2]复旦大学生命科学院立人实验室

出　　处：《第二军医大学学报》1998年第S1期44-46,共3页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金;国家自然科学基金委员会杰出人才基金;上海市卫生系统百名跨世纪优秀学科带头人培养计划资助

摘　　要：目的：观察烧伤血清（ＢＳ）对成年大鼠分离的心肌细胞膜钙通道电流的影响，探讨烧伤引起心肌细胞钙稳态变化的机制。方法：伤后２，６ｈ分离３０％ＴＢＳＡ（Ⅲ度）烧伤的ＳＤ大鼠血清；逆行循环灌流、胶原酶消化分离心肌细胞；膜片钳技术全细胞记录模式测定细胞膜钙通道电流。结果：２ｈＢＳ使峰值Ｌ型钙电流（ＩＣａ－Ｌ）比对照组增加５０％～８０％；而６ｈＢＳ使峰值ＩＣａ－Ｌ增加１．５～２．５倍；峰值电压－电流曲线显示烧伤血清明显增加各个去极化钳制电压下的ＩＣａ，并使得最大激活电压提前；细胞外液灌洗可去除烧伤血清对钙电流的作用。结论：烧伤血清能显著升高心肌细胞膜上钙通道电流，Ｌ型钙通道开放是烧伤后心肌细胞钙超载的机制之一。Objective: To determine the effect of postburn serum(BS) on Ltype calcium currents in isolated cardiac myocytes and to evaluate the mechanism of the alterations of calcium homeostasis after severe burn injury. Methods: Serum was gathered from 30%TBSA fullthickness burned rats 2,6 h postburn. Cardiac myocytes were isolated by enzyme digestion using retrograde perfusion apparatus. Ltype calcium (ICaL) currents were recorded in the wholecell patchclamp model. Results: ICaL were significantly increased by the stimulation of 2 h　BS and 6 h　BS, with the rate of 50%～80% and 1.5～2.5 folds respectively, and the VI curves were significantly downregulated, which could be completely washed out. Conclusion:Burn serum could stimulate the increase of ICaL and the activation of calcium channel might be a cellular mechanism of calcium overload of cardiomyocytes after severe burn injury. 

关 键 词：烧伤 心肌 钙通道.L型 膜片钳术 

分 类 号：R644.02[医药卫生—外科学]