肠毒素的生物电化学检测  

The Bioelectrochemical Assay for SEC

作　　者：董飒英[1,2] 刘馨苗 吕鸣祥[1,2] 田波义[1,2] 

机构地区：[1]725研究所青岛分部 [2]天津大学应用化学系

出　　处：《卫生研究》1998年第S1期117-119,共3页Journal of Hygiene Research

基　　金：国防科工委基金

摘　　要：论述了用电化学方法检测肠毒素的酶免疫电极，它兼有酶电极和免疫电极的特点，而酶电极的研究已较为成熟，免疫电极的电化学检测九十年代中期才刚刚兴起，正引起人们的广泛关注。实验中基底材料为玻碳电极，玻碳电极经过打磨抛光，超声清洗，再耦联生物分子。文中比较了用共价结合法，吸附法和电沉积法制备的生物传感器的响应特性，从实验结果可知镀有铂黑的生物电极，其表面积大大提高，酶、抗体等生物分子的结合量增多，因而检测能力也就随之增强。另一优点是信噪比提高，抗干扰能力加强。但缺点是重现性不好。电沉积法制备的电极重现性较好，但响应电流较小。おhis paper describes the bioelectrochemical assay for SEC by enzymeimmunoelectrode, which has the characters of both enzyme electrode and immunoelectrode. The research of enzyme electrode has been concluted for a long time, but the electrochemical assay of immunoelectrode has only begun from the 1990 and has attracted much attention.In this experiment, the basic material was glass carbon electrode(GC). Before using, a GC electrode was polished and treated in an ultrasonic cleaner. The response curves of biosensors were prepared by covalent binding, adsorption and electrodeposition. The results indicate that the platinized electrode has larger surface area and more enzyme and antibody, therefore, this kind of electrode has better test capability. Another advantage of the platinized electrode is better s/n rate and antiinterference character. But its disadvantage is bad repetition. The electrode prepared by electrodeposition has good repeated ability, but its response current is smaller. In short to make sensitive and stable biosensor is the good of future research.

关 键 词：酶免疫电极 镀铂黑 响应电流 肠毒素 

分 类 号：R991[医药卫生—毒理学]