骨骼肌肌浆网在收缩潜伏期内的超微结构形态变化  

Ultrastructural change of sarcoplasmic reticulum in skeletal muscle during contraction latency. Journal of Zhejiang Agricultural University

作　　者：姜永良[1] 杨勇骥[1] 郑尊[1] 邵晓良[1] 夏金辉[1] 

机构地区：[1]第二军医大学基础部生物物理研究所

出　　处：《浙江大学学报（农业与生命科学版）》1998年第S1期7-10,共4页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：国家自然科学基金

摘　　要：采用双向红外线探测器－计算机控制的电刺激与超低温快速冷冻固定同步技术对被电刺激后的骨骼肌作快速冷冻固定，采用透射电镜对电刺激后０．８ｍｓ，５．６ｍｓ，８．４ｍｓ及完全舒张时的蟾蜍腓肠肌超微结构变化进行了对比研究。骨骼肌组织在电刺激后０．８ｍｓ，５．６ｍｓ及８．４ｍｓ时发现肌浆网的前端，膜产生两个圆孔，而在骨骼肌处于全舒张时，肌浆网膜并无此形态结构。骨骼肌兴奋－收缩偶联发生时，发现肌浆网近Ｔ－管端的膜产生两个圆孔，而骨骼肌处于全舒张时，肌浆网膜也无此形态结构；骨骼肌收缩时出现的肌浆网膜小孔是否与肌浆网内某种物质（引起钙离子释放的某种未知物质？钙流？）在肌组织收缩时的转运或释放有关？This paper reports the ultrastructural morphological changes with the millisecond time course in skeletal muscle during excitation contraction coupling. The skeletal muscular ultrastructure were obtained at known millisecond time course following quick freezing after a single electrical stimulation.The one result shows that the substance of electronic concentration,in the SR,shift in front the SR facing the T tube during the time course of 0.8 ms to 10 ms (latency)。 Another result is that there two holes on the plasmalemma of the front of SR(facing the T tube)after electrical stimulation during the time course of 0.8 ms to tetanus。

关 键 词：骨骼肌 兴奋-收缩偶联 快速冷冻固定 

分 类 号：Q24[生物学—细胞生物学]