人表皮细胞的体外培养  

CULTURE OF HUMAN EPITHELIUM IN VITRO

作　　者：朱家源[1] 利天增[1] 苏爱云[1] 郭浩光[1] 唐庆[1] 朱斌[1] 

机构地区：[1]中山医科大学附属第一医院烧伤外科

出　　处：《中山大学学报（医学科学版）》1998年第S1期38-40,共3页Journal of Sun Yat-Sen University:Medical Sciences

摘　　要：目的：对表皮细胞在不同的培养方法、不同的培养液及不同底物中的生长情况进行３方面的探讨，以便选出较好的一种组合进行表皮细胞培养，为改善烧伤皮源不足的问题打下基础。方法：①采用人包皮或体皮制成皮块、皮粒、表皮细胞悬液进行液体培养，比较３种方法中表皮细胞生长情况；②应用纯表皮细胞悬液进行空气液体界面培养，平铺平皿培养及培养瓶液体培养，比较表皮细胞贴壁、生长情况；③另将表皮细胞悬液接种于３种不同的培养液，即ＲＰＭＩ１６４０，ＲＰＭＩ１６４０＋ＭＥＭ及ＭＥＭ比较细胞生长差异。结果：①在适当的条件下，离体的皮肤皮块、皮粒及表皮细胞悬液经培养皆能生长和分化，而表皮细胞悬液生长速度较快，纤维母细胞污染机会最少；②以Ｂｉｏｆｉｌ为底物的空气液体界面法，细胞生长速度最快，培养出的单层表皮细胞膜片易取出，不收缩；③表皮细胞在ＭＥＭ，ＲＰＭＩ１６４０及ＲＰＭＩ１６４０＋ＭＥＭ３种培养基中的生长情况基本一致。结论：以Ｂｉｏｆｉｌ为底物的空气液体界面培养法应得到推广应用，它不仅加快了表皮细胞的生长速度，减少了纤维母细胞的污染，易于移植。Objective: To investigate the growing conditions of epidermal cell cultured with different methods, different cultivation liquids and different substrates, so as to choose the best one. Methods:①Skin fragment, microskin and cell suspension of human epithelium were cultured, and the growing conditions were compared. ②Culturing effects of airliquid interface, flatmembrane method and liquid method were compared.③ Culturing effects of different cultivation liquids were compared. Results:①In suitable conditions, three type of epithelium all could grow and differentiate, but the cell suspension growing the fastest and having the least probability of being contaminated with fibroblasts.②The epidermal cell in airliquid interface growing faster than in the other interface, the single layer epithelial sheet cultured from Biofill was easily detached from dish and didn't shrunk. ③The culturing effects of different cultivation liquids were same. Conclusions:The method of using Biofill as substrate withairliquid interface would be widely used for its fast growth rate, free from fibroblast, transplanted simply and its probability of being used in the third degree burn for a long time.

关 键 词：表皮细胞 组织培养 表皮 

分 类 号：Q2-33[生物学—细胞生物学]