草河车对DHBV感染雏鸭急性肝坏死的保护作用  

Protective Effect of Paris Polyphylla Smith on Acute Hepatic Failure of Ducklings Infected Duck Hepatitis B Virus

作　　者：任园[1] 陈湘红[2] 陈鸿珊[2] 周立[2] 

机构地区：[1]中国医学科学院肿瘤研究所,北京100021 [2]中国医学科学院医药生物技术研究所

出　　处：《中国中西医结合杂志》1998年第S1期161-163,378,共4页Chinese Journal of Integrated Traditional and Western Medicine

摘　　要：目的:评价草河车对鸭乙型肝炎病毒(DHBV)感染雏鸭急性肝坏死的作用。方法:用脂多糖(LPS)诱发 DHBV 感染雏鸭急性肝坏死,建立实验模型。腹腔注射200mg·kg^(-1)·d^(-1)草河草,治疗过程中观察鸭血清丙氨酸氨基转移酶(ATL)、肿瘤坏死因子(TNF)和 DHBV-DNA 的动态水平,并进行鸭肝病理检查。结果:LPS 能诱发 DHBV 感染35天鸭急性肝坏死。草河车给药组 LPS 不能诱发鸭肝坏死。给药组在第2次注射 LPS 后9、18、24、48和72h,与未给药组同期比较,血清 ALT 水平显著降低,血清 TNF 水平也明显下降,但血清 DHBV-DNA 水平无明显变化。结论:草河车对 DHBV 感染雏鸭急性肝坏死具有显著性保护作用。Objective:To evaluate the effect of Paris polyphylla ,Smith (PPS) on acute hepatic failure of ducklings infected duck hepatitis B virus (DHBV).Methods:An acute hepatic failure model was established in ducklings infected DHBV with lipopolysaccharides (LPS).LPS-challenged ducklings were injected intraperitoneally with 200 nag.kg^(-1) PPS per day.Dy- namic levels on alanine aminotransferase (ALT),tumor necrosis factor (TNF) and DHBV-DNA in sera of the duckings were determined.Liver pathologic detections of the ducklings were carried out.Results:LPS caused acute hepatic failure of ducklings on day 35 after DHBV infection,but not on day 14.Compared with the control group,acute hepatic failure did not occur in the drug-treated group.PP treatment could markedly decrease the levels of ALT and TN-F in sera of ducklings at hours 9,18,24,48 and 72 after LPS injection.Conclusion:PP could protect effectively ducklings infected DHBV against LPS-mediated acute hepatic failure.

关 键 词：草河车 鸭乙型肝炎病毒 急性肝坏死 

分 类 号：R285.5[医药卫生—中药学]