益智胶囊对血管性痴呆动物模型影响的实验研究  

The Experimental Study of the Effect of Yizhi Capsule(益智胶囊)in the Animal Model of Vascular Dementia

作　　者：陈楷[1] 陈可冀[1] 张国玺[1] 周文泉[1] 涂人顺[1] 刘丽[1] 

机构地区：[1]中国中医研究院老年医学研究所,北京100091

出　　处：《中国中西医结合杂志》1998年第S1期219-221,388,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家中医药管理局八五攻关课题

摘　　要：目的:探讨益智胶囊对血管性痴呆动物的作用机理。方法:采用左心室注射液体石蜡引发多发性脑梗塞造成动物血管性痴呆模型的方法。造模24h 后,将动物分为对照组、模型组、治疗大、中、小剂量组及喜得镇组。治疗组给予益智胶囊药粉,剂量分别为0.32、0.64、1.28g/kg;喜得镇组给予喜得镇为0.857mg/kg;对照组及模型组给予生理盐水。各组动物连续给药14天。学习和记忆能力测定采用避暗法和穿梭箱法,测定血液流变学指标,脑切片做 HE 和 TTC 染色,并应用显微图像分析仪计算脑梗塞面积和海马 CA_1区单位面积的锥体细胞数。结果:益智胶囊能提高模型动物的学习记忆能力,脑梗塞面积减少,脑海马单位面积的锥体细胞数量及损伤程度较模型组减轻。结论:益智胶囊确有一定的改善模型动物学习的作用。Objective:To study the effective mechanism of Yizhi capsule(YZC)on the animal model of vascular dementia. Methods:The rats were used for the model,which induced by injected olefin oil from left ventricle causing cerebral multi- ischemic infarcts.Twenty-four hours after the model was made,the rats were divided into control,model,treatment and Hydergine groups.The YZC dosages were given 0.32 g/kg,0.64 g/kg,1.28 g/kg to the small,medium and large dosage groups.The Hydergine was given 0.857 mg/kg.The control and model groups were given 0.9% sodium solu- tion.The drugs were given 14 days consecutively.The tests of step down and shuttle box were used to assess the animal learning and memory abilities.HE and TTC staining were taken.The biological medical picture analysis system was used to calculate the infarctional area and pyramidal cells on the Hippocampal CAI.Results:The YZC improved the learning and memory abilities of the model animals.The infarctional areas were significantly smaller than that of model group.The damage of Hippocampal CAI were lighter than that in the model group.Conclusion:The YZC had certain effect of im- proving the learning and memory abilities of the model.

关 键 词：益智胶囊 血管性痴呆 动物模型 

分 类 号：R285.5[医药卫生—中药学]