感染抗青蒿酯钠伯氏疟原虫株小鼠血清对青蒿酯钠抗疟药药效的拮抗作用  

The Antagonism of the Serum of Mice Infected with Artesunate-resisting Piasmodium Berghei against the Antimalarial Action of Sodium Artesunate

作　　者：刘爱如[1] 吕丽莉[1] 隋在云[1] 

机构地区：[1]山东省中医药研究所,济南250014

出　　处：《中国中西医结合杂志》1998年第S1期329-330,406-407,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家中医药管理局科研基金资助课题

摘　　要：目的:研究疟原虫抗青蒿酯钠的产生机理。方法:分别采用感染抗青篙酯钠伯氏疟原虫株小鼠血清(SM SA-R)、感染青蒿酯钠敏感株伯氏疟原虫小鼠血清(SM SA-S)和无原虫感染的正常小鼠血清(SM)与青蒿酯钠(SA)水溶液的混合物治疗感染正常伯氏疟原虫小鼠,于给药后3天(d3)、4天(d4)和5天(d5)涂片检查其感染率。结果:SM SA-R 加 SA 组、SMSA-S 加 SA 组和 SM 加 SA 组的原虫感染率分别为:d3:(16.8±1.7)%、(10.3±2.5)%、(9.0±2.0)%,d4:(28.6±5.0)%、(20.9±5.2)%、(17.6±4.4)%,d5:(39.2±8.5)%、(27.0±7.1)%、(23.1±5.4)%。SM SA-R 加 SA 组与其他两组原虫感染率比较,差异显著(P<0.01)。结论:SM SA-R 对 SA 抗疟药药效具有拮抗作用。提示 SA-R 疟原虫可能产生了某种“特异性抗药物质”。Objective:To Study the artesunate-resisting effect produced in Plasmodium berghei(Pb).Methods:Sodium arte- sunate(SA)solution was mixed separatedly with the serum of mice infected with SA-resisting(SA-R)Pb strain,the serum of mice infected with SA-sensitive(SA-S)Pb strain and the serum of normal mice(SM).These mixtures were used in treating mice inoculated with Pb normal strain.The erythrocyte infection rates in the above-mentioned mice were determined on the 3rd(d3),4th(d4)and 5th(d5)day after drug-serum administration.Results:The erythrocyte-infec- tion rates(%),in the SM SA-R+SA,SM SA-S+SA and SM+SA groups obtained on d3,d4 and d5 after drug-serum administration were d3:16.8±1.7,10.3±2.5 and 9.0±2.0 repectively,d4:28.6±5.0,20.9±5.2,and 17.6±4. 4 repectively,d5:39.2±8.5,27.0±7.1 and 23.1±5.4 respectively.There were significant differences with respect to parasitemia between the SM SA-R+SA group and the SM SA-S+SA or SM+SA group,P<0.01.Conclusions:SM SA-R,nay be antagonistic to the antimalarial action of SA.SA-R Plasmodia might produce a specific anti-drug sub,stance, leading to the antagonism mentioned above.

关 键 词：微生物抗药性 伯氏疟原虫正常株 伯氏疟原虫抗青蒿酯钠株 青蒿酯钠 

分 类 号：R285.5[医药卫生—中药学]