哺乳类细胞基因表达系统  

Mammalian cell gene expression system

作　　者：孙蕾[1] 王会信[1] 

机构地区：[1]军事医学科学院基础医学研究所,北京100850

出　　处：《生物技术通讯》1998年第1期65-67,71,共4页Letters in Biotechnology

摘　　要：真核基因的转录和转译调控是哺乳类细胞基因表达系统建立和发展的基础,外源基因的表达水平不仅决定于启动子/增强子的强弱,还与剪接信号、终止信号和poly(A)信号以及质粒的拷贝数等因素有关。另外,在基因治疗的研究中,也已寻找到多种具有组织或肿瘤特异性的启动子,来达到特异性肿瘤基因治疗的目的。Transcription and translation regulation of eukaryotic cell is foundamental for the construction and development of mammalian cell gene expression system. The expression level of foreign gene was decided by both the strength of promoter/enhancer and other elements, such as splicing signal, stopping signal and copies of the plasmid. Whatsmore, many .specific promoters have also been found to achieve tissue-specific and tumor-specific gene therapy.

关 键 词：哺乳类细胞基因表达 启动子/增强子 剪接信号 质粒拷贝数 

分 类 号：Q75[生物学—分子生物学]