粳稻花药培养技术的研究初报  

Study on Technique of Anther Culture in Japonica Rice

作　　者：金石芬[1] 张增明[1] 刘金明[1] 

机构地区：[1]吉林市农科院

出　　处：《延边大学农学学报》1998年第1期30-31,共2页Agricultural Science Journal of Yanbian University

摘　　要：在粳稻花药培养过程中，花粉植株的诱导频率受接种材料的基因型、花粉发育时期和培养基成分等因素的影响．杂交种的诱导率高于纯合品种，接种最佳时期为花粉发育单核中晚期，诱导培养基以Ｎ６＋２．４—Ｄ２ｍｇ／ｌ＋ＫＴ０．５ｍｇ／ｌ＋蔗糖５％＋琼脂粉０．７％；分化培养基以Ｍｓ＋ＢＡ２ｍｇ／ｌ＋ＮＡＡ０．２—０．５ｍｇ／ｌ＋蔗糖３％＋琼脂粉０．７％；壮苗培养基以１／２ＭＳ＋微量＋铁盐＋ＮＡＡ０．１—０．２ｍｇ／ｌ＋多效唑２ｍｇ／ｌ＋蔗糖２％＋琼脂０．In the anther culture of Japonica Rice,the induction rate of pollen plantlets was affected by the genotypes of materials, developmental stage of anthers,formulae of culture medium and so on. Hybrids had a higher induction rate as compared with homozygous varieties.The suitable developmental stage of the microspores in the anthers was in the mid-or-late-uninucleate.Better medium formulae were N6+2mg/l2.4-D+0.5mg/l KT+5% sucrose+0.7% agar for dedifferentiation and Ms+2mg/lBA+0.2-0.5mg/l NAA+3% sucrose+0.7%agar for differentiation.To make the plantlets strong,you should transfer them onto 1/2 Ms (allFe-salt)+0.1-0.2mg/l NAA+2mg/l MET+2% sucrose+0.7% agar.

关 键 词：粳稻 花药 培养效率 

分 类 号：S511.220.98[农业科学—作物学]