Mapping epitopes of human papillomavirus type 16 L1 protein with a phage display epitope library  

作　　者：刘天菊 司履生 王一理 孙向乐 杨居祥 耿宜萍 

出　　处：《Chinese Medical Journal》1998年第3期11-11,共1页中华医学杂志（英文版）

摘　　要：Abstract Objective It has been well accepted that human papillomavirus type 16 (HPV16) is associated with human cervical cancer and HPV16 L1 protein could induce both humoral and cellular immune responses. The objective of this study is to map epitopes on HPV16 L1 protein and to provide information to the design of HPV16 prophylactic peptide vaccine. Methods The epitopes on L1 protein were screened by polyclonal and 2 monoclonal antibodies (B8 and F4G3) against HPV16 L1 protein from a 6 mer phage display epitope library with the method of immuno affinity screening (Bio panning). After 3 rounds of Bio panning, the positive phages were detected by L1 antibodies again with ELISA. The positive phages re acted strongly with L1 antibodies were then identified Department of Immunopathology, Xi'an Medical University, Xi'an 710061, China (Liu TJ, Si LS, Wang YL, Sun XL, Yang JX and Geng YP) This study was supported by China Medical Board in America (CMB). by DNA sequencing. Results Three mimotopes have been screened by polyclonal and two monoclonal antibodies. The mimotope (LSLFSC) which reacted with monoclonal antibody B8 showed 50% homology with the sequence 270～275a.a (DSLFFY) of prototype HPV16 L1. Another mimotope (LTSSYS) which reacted with polyclonal antibodies had 66% homology with the L1 sequence 516～521a.a (TTSSTS), also a mimotope (DRWDRF) was found to have the homologic RF with the known L1 sequense 441～446a.a. The mimotopes LSLFSC and DRWDRF were adjacent to the epitopes at 267～269a.a and 422～441a.a reported by other researchers previously. Conclusions Our results suggested that there might be a batch of epitopes on HPV16 L1 protein, and the predominant epitopes of HPV16 L1 protein were located in the above two domains. These results would be heplful for the design of HPV16 prophylactic peptide vaccines and HPV polyvalent vaccines.Abstract Objective It has been well accepted that human papillomavirus type 16 (HPV16) is associated with human cervical cancer and HPV16 L1 protein could induce both humoral and cellular immune responses. The objective of this study is to map epitopes on HPV16 L1 protein and to provide information to the design of HPV16 prophylactic peptide vaccine. Methods The epitopes on L1 protein were screened by polyclonal and 2 monoclonal antibodies (B8 and F4G3) against HPV16 L1 protein from a 6 mer phage display epitope library with the method of immuno affinity screening (Bio panning). After 3 rounds of Bio panning, the positive phages were detected by L1 antibodies again with ELISA. The positive phages re acted strongly with L1 antibodies were then identified Department of Immunopathology, Xi'an Medical University, Xi'an 710061, China (Liu TJ, Si LS, Wang YL, Sun XL, Yang JX and Geng YP) This study was supported by China Medical Board in America (CMB). by DNA sequencing. Results Three mimotopes have been screened by polyclonal and two monoclonal antibodies. The mimotope (LSLFSC) which reacted with monoclonal antibody B8 showed 50% homology with the sequence 270～275a.a (DSLFFY) of prototype HPV16 L1. Another mimotope (LTSSYS) which reacted with polyclonal antibodies had 66% homology with the L1 sequence 516～521a.a (TTSSTS), also a mimotope (DRWDRF) was found to have the homologic RF with the known L1 sequense 441～446a.a. The mimotopes LSLFSC and DRWDRF were adjacent to the epitopes at 267～269a.a and 422～441a.a reported by other researchers previously. Conclusions Our results suggested that there might be a batch of epitopes on HPV16 L1 protein, and the predominant epitopes of HPV16 L1 protein were located in the above two domains. These results would be heplful for the design of HPV16 prophylactic peptide vaccines and HPV polyvalent vaccines.

分 类 号：R373.9[医药卫生—病原生物学]