Molecular cloning, sequence analysis and expression studies of a novel GAmyb homologous gene, hvmyb  

作　　者：凌世昀 祁放 朱玉贤 

出　　处：《Science China(Life Sciences)》1998年第5期525-529,共5页中国科学（生命科学英文版）

基　　金：EMBLaccessionnumberY14 6 5 8.

摘　　要：Using a known GAmyb gene as the probe, two fully identical clones were isolated from a barley aleurone cDNA library. Sequence analysis showed that their 5′ termini are highly homologous to the 3′ termini of GAmyb (97%) and their 3′ termini share no significant homology with any myb genes. Therefore, the deduced protein may hold intact putative GAmyb activation domain but lack the normal DNA binding domain. Northern blot reveals that hvmyb expression in barley aleurone layers is strongly up regulated by gibberellin (GA) and down regulated by abscisic acid (ABA). The tissue and developmental stage specificity of hvmyb was also found, which was only expressed in barley aleurone cells and dropped to non detectable level soon after germination.Using a known GAmyb gene as the probe, two fully identical clones were isolated from a barley aleurone cDNA library. Sequence analysis showed that their 5′ termini are highly homologous to the 3′ termini of GAmyb (97%) and their 3′ termini share no significant homology with any myb genes. Therefore, the deduced protein may hold intact putative GAmyb activation domain but lack the normal DNA binding domain. Northern blot reveals that hvmyb expression in barley aleurone layers is strongly up regulated by gibberellin (GA) and down regulated by abscisic acid (ABA). The tissue and developmental stage specificity of hvmyb was also found, which was only expressed in barley aleurone cells and dropped to non detectable level soon after germination.

关 键 词：BARLEY ALEURONE GAMYB GA ABA. 

分 类 号：Q78[生物学—分子生物学]