Photocycle of 13-cis-retinal in bacteriorhodopsin caused by destruction to the cooperative effect of purple membrane  

作　　者：胡坤生 何靳安 张衡涛 

出　　处：《Science China Chemistry》1998年第2期135-141,共7页中国科学（化学英文版）

基　　金：ProjectsupportedbythekeyandmajorprojectsfromtheChineseAcademyofSciences (GrantNos.Kj95 1 Al 5 0 1 0 5andKj95 2 S1 0 3)

摘　　要：The steady absorption and kinetic changes of M 412 intermediate of the light and dark adapted bacteriorhodopsin (BR) solubilized by different concentrated Triton X 100 were investigated. The results indicated that the cooperative effect existing within the trimeric BR of native purple membrane (PM) was damaged in the system containing the surfactant since the component and structure of the bilayer lipid membrane in PM varied due to the solubilization of partial PM lipids by Triton X 100. The destruction to the cooperative effect of BR ultimately caused 13 cis retinal of the dark adapted BR to take part in BR photocycle and also to generate the deprotonated M 412 intermediate.The steady absorption and kinetic changes of M412 intermediate of the light- and dark-adapted bacteriorhodopsin (BR) solubilized by different concentrated Triton X-100 were investigated. The results indicated that the cooperative effect existing within the trimeric BR of native purple membrane (PM) was damaged in the system containing the surfactant since the component and structure of the bilayer lipid membrane in PM varied due to the solubilization of partial PM lipids by Triton X-100. The destruction to the cooperative effect of BR ultimately caused 13-cis-retinal of the dark-adapted BR to take part in BR photocycle and also to generate the deprotonated M412 intermediate.

关 键 词：BACTERIORHODOPSIN PURPLE membrane cooperative effect TRITON X 100 M 412 intermediate. 

分 类 号：Q63[生物学—生物物理学]