Energy transfer kinetics of phycoerythrocyanin trimer from cyanobacterium Anabaena variabilis (II)  

Energy transfer kinetics of phycoerythrocyanin trimer from cyanobacterium Anabaena variabilis (II)

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作  者:张景民 赵井泉 蒋丽金 陈建新 叶彤 张启元 

出  处:《Science China(Life Sciences)》1998年第2期133-138,共6页中国科学(生命科学英文版)

基  金:ProjectsupportedbytheNationalNaturalScienceFoundationofChina (GrantNo .396 70 0 6 5 ) .

摘  要:The excitation energy transfer processes in trimeric PEC have been studied by using steady state and time resolved fluorescence spectra techniques in detail. The results indicate that the energy transfer processes should take place between α 84 PVB and β 84 or β 155 PCB chromophores with the time constants 34.7 ps and 175-200 ps individually; in contrast with monomeric PEC, from time resolved fluorescence anisotropic spectrum technique, the decay constant of 45 ps which was assigned to the energy transfer time among three β 84 PCB chromophores was observed and the energy levels of β 84 and/or β 155 PCB chromophores were confirmed to turn over in trimeric PEC.The excitation energy transfer processes in trimeric PEC have been studied by using steady state and time resolved fluorescence spectra techniques in detail. The results indicate that the energy transfer processes should take place between α 84 PVB and β 84 or β 155 PCB chromophores with the time constants 34.7 ps and 175-200 ps individually; in contrast with monomeric PEC, from time resolved fluorescence anisotropic spectrum technique, the decay constant of 45 ps which was assigned to the energy transfer time among three β 84 PCB chromophores was observed and the energy levels of β 84 and/or β 155 PCB chromophores were confirmed to turn over in trimeric PEC.

关 键 词:PHYCOBILISOME PHYCOERYTHROCYANIN DECONVOLUTION spectrum time resolved fluorescence energy transfer. 

分 类 号:Q937[生物学—微生物学]

 

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