牛蛙前庭毛细胞A型钾离子流的实验研究  

A-type potassium currents in bull-frog vestibular hair cells:An experimental study

作　　者：沙建慧[1] 李云义[1] 李云阁[1] 刘焕珠[1] 张文杰[2] 战术[2] 

机构地区：[1]空军医学高等专科学校,吉林市132013 [2]白求恩医科大学,长春市130021

出　　处：《空军医高专学报》1998年第3期125-128,共4页

摘　　要：为了进一步探讨前庭细胞膜上通道的类型及动力学特征,采用全细胞式膜片钳技术在牛蛙前庭毛细胞侧膜上研究了钾通道电流。结果表明阻断内向电流后,当钳制电位为-100mV,阶跃从-70mV至+20mV时,随着膜电位的去极化,可记录到快速瞬时的A型通道电流,激活电压为（-53±13）mV,4-Ap对其有特异性阻断作用;A型钾通道的数量在牛蛙毛细胞间存在差异;除A型钾通道外,细胞膜上还含有其它类型的通道,这种通道能被TEA阻断,其性质待定。In order to further explore the type and kinetic feature of potassium channel on the vestibular hair cell membrane, whole-cell patch -clamp technique was used to investigate potassium current on the bull-frog's vestibular hair cell membrane in this experiment. The experimental result showed: when holding potential was - 100 mV, rapid and instant current of A-type potasium channel could be recorded by the depolarizing voltage pulse from - 70 mV to + 20 mV and could be blocked, especially by 4-Ap. The activated membrane potential was (- 53±13)mv. The amount of the A-type potassium channels was different among various hair cells. Besides A-type potassium channels, there were others potassium channels on the hair cell membrane. They could be blocked by TEA. The nature remained to be further investigated.

关 键 词：牛蛙 前庭 毛细胞 钾离子流 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]