Ⅳ型胶原放射免疫分析方法的建立及临床应用  

Study of type IV collagen radioimmunoassay kit and its clinical application

作　　者：姚敏捷[1] 黄汉朝[1] 袁忠海[1] 郭素红[1] 汤勇[2] 那爱华[2] 

机构地区：[1]空军医学高等专科学校,吉林市132013 [2]空军总医院,北京市100036

出　　处：《空军医高专学报》1998年第2期85-88,共4页

摘　　要：采用单克隆技术制备鼠抗人Ⅳ型胶原（Ⅳ-C）单克隆抗体,用氯胺-T方法标记,应用免疫竞争法建立Ⅳ-C放射免疫分析方法,其检测范围20μmg/L～500μmg/L。其方法通过特异性、精密度、准确性、亲和常数等实验方法学检验,表明Ⅳ-C放射免疫分析方法符合临床应用要求。应用该方法检测30例肝炎患者、15例肝硬化患者、35例Ⅱ型糖尿病（NIDDM）患者、25例甲亢患者,除NIDDM、甲亢患者外,其余患者血清浓度明显高于正常人（P<0.01）;NIDDM、甲亢患者Ⅳ-C血清浓度高于正常人（P<0.05）。结论Ⅳ-C血清浓度检测在肝病、肾病及相关疾病的研究中有一定的临床价值。Objective: To found a radioimmunoassay (RIA) method of Type Ⅳ Collagen(Ⅳ-C) for clinic applications.Method: Mouse monoclonal Ⅳ-C antibody were prepared by monclonal technology.Ⅳ-C were labelled the chloramine-T method.Results:Method of detection Ⅳ-C was deveolpedn by RIADection range:20-500ug/L. Conclusion: The RIA of Ⅳ-C was tested by sensitivity,affinity constant,sensitivity,precision and the results indicate that RIAof Ⅳ-C fits clinic applications. Concenfrations of serum Ⅳ-Cwere measured by RIA in 30 cases hepatitis and 15 cases cirrhosis of liver and 35 cases hepatitisand 15 cases hperthyroidism and compared with those in 50cases normal humans, the concertrations of serum Ⅳ-C in all patients were markedly increased iespectivedly expect NIDDM and and and hyperthyroidism were significantly higher than normal humsns(P<0.05). These results suggest that serum Ⅳ-Ceoncentration is valuble in clinical study of liver and other relative diseases.

关 键 词：Ⅳ型胶原 放射免疫法 单克隆抗体 

分 类 号：R392[医药卫生—免疫学]