猪SR-BⅠ基因的克隆及其组织差异表达规律研究  

Cloning and differential expression of SR-BⅠgene in pigs

作　　者：堵斌[1] 孙超[1] 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100

出　　处：《西北农林科技大学学报（自然科学版）》2009年第4期7-12,共6页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家自然科学基金项目(30471267);教育部新世纪优秀人才支持计划项目(NCET-06-0865)

摘　　要：【目的】扩增猪各组织清道夫受体B族Ⅰ型(SR-BⅠ)基因片段,并对其进行生物信息学分析,研究SR-BⅠ在猪中的表达规律。【方法】采取不同月龄和不同猪种猪的内脏脂肪、皮下脂肪、肝脏、脾脏、肌肉、肾脏、肺脏、心脏等组织,提取其RNA,根据GenBank已发表的人、小鼠及大鼠SR-BⅠ基因序列,设计并合成1对引物,用RT-PCR法扩增猪SR-BⅠ基因cDNA片段,检测该基因在猪各组织中的分布及其在不同月龄、不同部位、不同猪种脂肪组织中的转录表达规律。【结果】成功克隆了猪SR-BⅠcDNA片段,长度为192 bp(EU366320);该基因与牛、人、中国仓鼠、大鼠、小鼠在氨基酸序列上分别有84.1%,78.6%,77.5%,67.0%和66.6%的同源性;SR-BⅠ基因mRNA在瘦肉型猪种上的表达量显著高于脂肪型猪种,随月龄的增加其表达量逐渐上升,且其在皮下脂肪的表达量较内脏脂肪高。【结论】SR-BⅠmRNA的表达与猪肥胖程度、年龄以及脂肪沉积部位密切相关。[Objective] eDNA sequences of the SR-BⅠ gene were obtained and analyzed in biologic information,based on which the expression of SR-BⅠ in pigs was studied. [Method] Visceral fat,subcutaneous fat, liver, spleen, muscle, kidney, lung and heart of different growth-stages and different types of pigs were collected. A pair of PCR primers were designed and synthesized according to SR-BⅠ gene sequence of human,mouse and rat. Total RNA was extracted from adipose tissue of pigs,and then the SR-BⅠ gene eDNA was cloned by RT-PCR. Thereafter, the level of SR-BⅠ mRNA in various regions, different growthstage, different parts and different types of pig adipose tissue were measured. [Result] The sequenceing results showed that 192 bp pig＇s SR-BⅠ eDNA fragment（EU366320） was successfully cloned. Homologous comparison with some animals indicated that pig SR-BⅠ cDNA shared 84. 1%, 78.6%, 77.5%, 67.0%, 66.6% similarity in bovine, human,cricetulus griseus,mouse and rat. The expression level of SR-BⅠ mRNA was significantly higher in adipose tissue of lean pigs than that of obese pigs, and also the expression level gradually increased as age grew. It was found that the abundance of SR-BⅠ mRNA level increased more in subcutaneous fat than in visceral fat. [Conclusion] Our result suggested that SR-BⅠ is a potential factor which may regulate adipogenesis in adipose tissue.

关 键 词：猪 SR-BⅠ 脂肪组织 表达规律 

分 类 号：S828.2[农业科学—畜牧学] Q78[农业科学—畜牧兽医]