SW-BSA偶联率对小鼠免疫原性的影响  

Effects of the conjugate ratio of SW-BSA on the immunogenicity in mice

作　　者：宋毓民[1] 王爱华[1] 王建华[1] 唐金宁 赵兴华[1] 余永涛[1] 李海利[1] 耿果霞[1] 

机构地区：[1]西北农林科技大学动物医学院,陕西杨凌712100 [2]山东省临沂市动植物园,山东临沂276700

出　　处：《西北农林科技大学学报（自然科学版）》2009年第4期19-24,共6页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家自然科学基金项目(30571315和30871901);高等学校博士学科点专项科研基金项目(20030712017)

摘　　要：【目的】探讨合成苦马豆素人工抗原(SW-BSA)的不同偶联率对小鼠免疫原性的影响,筛选合成SW-BSA的最佳偶联率。【方法】苦马豆素(Swainsonine,SW)和载体牛血清白蛋白(BSA)分别以20∶1,30∶1,40∶1和50∶1的比例化学合成4种SW-BSA(分别为AgA、AgB、AgC和AgD),用紫外分光光度法分别测定SW与BSA的偶联率;分别用AgA、AgB、AgC和AgD免疫小鼠,免疫3次后,间接ELISA试验检测血清效价。【结果】合成的4种SW-BSA的偶联率分别为14.7,20.2,25.3和32.6;AgA、AgB、AgC和AgD各组免疫小鼠的血清抗体效价分别为214,>215,215和213,4种SW-BSA均能刺激小鼠产生高效价抗体,其中AgB组的抗体效价最高,与其他各组相比差异显著(P<0.05),AgA组和AgC组间差异不显著(P>0.05)。【结论】4种不同偶联率的SW-BSA对小鼠均具有较好的免疫原性,其中以偶联率为20.2的SW-BSA(AgB)的免疫原性最高。[Objective] The study explored the effects of different conjugate ratios of SW-BSA synthesized on the immunogenicity in mice to select the best conjugate ratio of synthesized SW-BSA. [Method] The four kinds of SW-BSA（AgA, AgB, AgC and AgD） were synthesized with swainsonine and carrier,bovine serum albumin（BSA）,with the proportion of 20 ： 1,30 ： 1,40 ： 1 and 50 ： 1,and the conjugate ratio was determined respectively by ultraviolet speetrophotometry. Then the mice were immunized with AgA, AgB, AgC and AgD, after 3 times, the blood serum titers were detected using indirect ELISA. [Result] The four binding ratios of SW-BSA were 14.7,20.2,25.3 and 32.6. The mice serum titers of AgA,AgB,AgC and AgD were 2^14 ,〉2^15 ,2^15 and 2^13 respectively,so the mice of four SW-BSA groups generated higher titers of antibody. Among them,was the highest the titer of group AgB,which had significant disparity compared with others（P〈0.05） ,at the same time the titers of group AgA and AgC had no significant disparity（P〉 0.05）. [Conclusion] The four couple rates of SW-BSA have better immunogenicity to mice, but the SW-BSA with the couple ratio of 20.2 is the highest.

关 键 词：苦马豆素人工抗原 化学合成 偶联率 免疫原性 

分 类 号：S852.5[农业科学—基础兽医学]