A　Study　on　the　Reaction　Mechanism　of　Bromocresol　Green　with　Bovine　Serum　Albumin  

作　　者：Zhu, K Li, KA Tong, SY 

机构地区：[1]BEIJING UNIV,DEPT CHEM,BEIJING 100871,PEOPLES R CHINA

出　　处：《Chinese Chemical Letters》1996年第11期1029-1032,共4页中国化学快报（英文版）

摘　　要：The protein assay with bromocresol green (BCG) method is commonly ed because of itsconvenience, but it is not well understood on a molecular level. In this paper, the study on thereaction between BCG and bovine serum albumin (BSA) is reported The binding numbers and thebinding conStants are given at different pH by using three methods with relevant comparison. It wasProved that there were three sorts of binding siteS of BSA to react with BCG and BCG transformedinto its boo form in the presence of BSA.The protein assay with bromocresol green (BCG) method is commonly ed because of itsconvenience, but it is not well understood on a molecular level. In this paper, the study on thereaction between BCG and bovine serum albumin (BSA) is reported The binding numbers and thebinding conStants are given at different pH by using three methods with relevant comparison. It wasProved that there were three sorts of binding siteS of BSA to react with BCG and BCG transformedinto its boo form in the presence of BSA.

关 键 词：REACTION MECHANISM the BOVINE Green Bromocresol 

分 类 号：O611.4[理学—无机化学]