甜瓜抗CMV转基因植株的分子生物学研究  

Molecular Biology of Gene-transfered CMV-resistant Honeymelon

作　　者：许键[1] 李仁敬[1] 孙严[1] 杨金菊[1] 喻梅辉[1] 

机构地区：[1]新疆农科院核生所

出　　处：《新疆农业大学学报》1995年第3期30-34,共5页Journal of Xinjiang Agricultural University

基　　金：国家自然科学基金

摘　　要：本研究建立了转目的基因植物基因整台及表达的分子生物学鉴定系统，并用报告基因检测、ＳＤＳ－ＰＡＧＥ、Ｄｏｔ－ＥＬＩＳＡ、ＰＣＲ特异扩增、Ｗｅｓｔｅｒｎ－Ｂｌｏｔ等方法，研究了用叶盘法经Ｔｉ质粒转化的甜瓜再生植株ＣＭＶ－ＣＰ基因的整合与表达。结果表明：再生植株基因组中整合了ＣＭＶ－ＣＰ基因，长度为１ｋｂ，并能有效表达。表达产物分子量为２４Ｋｄ，确为ＣＭＶ的外壳蛋白，其表达量占细胞总蛋白约５％。The system was established for the integration and expression assay of the purpose genes transfered into plants. Based on the techniques in this system,namely,reporter gene detection,SDS-PAGE, Dot-ELISA, PCR specific gene amplification and Western-Blot,such assay of CMV--Coat Protein (CP)gene,which had been transfered into honeymelon by Ti plaSmid using leaf-disc method,waS studied. The results showed that the Ikb CMV-CP gene did integrate with the genome of the regenerated plant. In addition,it had been ascertained that the expressed product of the gene was CMV-CP,with molecular weight of 24kd,which was produced efficiently to make up 5% of the total proteins in the gene-transfered honeymelon.

关 键 词：CMV-CP 转基因植物 分子生物学 

分 类 号：Q789[生物学—分子生物学]