Effects of glutamine on structure and function of gut in endotoxemic rats  

谷氨酰胺对败血症大鼠肠道结构和功能的影响

作　　者：秦环龙[1] 崔恒贵[1] 张彩华[1] 吴敦文 储祥平[1] 

机构地区：[1]镇江医学院附属医院普外科

出　　处：《World Journal of Gastroenterology》1996年第2期13+10-12,10-12,共4页世界胃肠病学杂志（英文版）

摘　　要：Abstract AIMS To study the effects of glutamine supplemented parenteral nutrition on protein metabolism, small intestinal mucosal morphology, and barrier function in endotoxin ureated rats. METHODS Thirty five male Wistar rats were divided into four groups randomly: group A, normal control, group B, enteral nutrition; group C, non_glutamine TPN; group D, glutamine TPN. Endotoxemia was induced by continuous intravenous infusion of endotoxin at a dose of 2mg/kg per day throught the 5 day study period. The small intestinal bacterial translocation rate, mucosal protein and DNA contents, and the contents of SOD, MDA, ATP and SIgA of mucosal homogenates were determined. Mucosal thickness and villous height were measured with light microscope, and thickness of microvillus were measured with electron microscope.目的本文旨在探讨谷氨酰胺对败血症大鼠小肠粘膜结构和功能的影响。方法３５只大鼠随机分为四组，Ａ组为正常对照组，Ｂ组为肠内营养组，Ｃ组为不含谷氨酰胺的肠外营养组，Ｄ组含有谷氨酰胺的肠外营养组。内毒素以２ｍｇ／Ｋｇ·ｄ的剂量，混入营养液或生理盐水中，持续滴注５ｄ。实验结束，测定小肠粘膜匀浆蛋白质，ＤＮＡ，ＳＯＤ，ＭＤＡ，ＡＴＰ，ＳＩｇＡ含量，光镜观察绒毛高度，小肠粘膜全层厚度，电镜观察微绒毛变化。结果含有谷氨酰胺的Ｄ组肠壁蛋白质和ＤＮＡ含量高于Ｃ组（Ｐ＜００１），明显降低了ＳＯＤ，ＭＤＡ含量（Ｐ＜００１），ＡＴＰ，ＳＩｇＡ含量在Ｄ组也明显升高（Ｐ＜００１），在绒毛高度、小肠粘膜及全层厚度等指标Ｄ组优于Ｃ组（Ｐ＜００１）。经肠道营养的Ｂ组在上述组织学观察数值上均与正常组相近，且粘膜厚度，绒毛高度均超过正常值。Ｂ、Ｄ二组肠道内细菌移位低于Ｃ组（Ｐ＜００１）。结论加入谷氨酰胺的肠外营养有助于防止败血症大鼠肠粘膜萎缩以及细菌移位。早期应用经肠道营养更具有积极的生理意义。

关 键 词：谷氨酰胺 胃肠外营养 败血病 小肠 细菌 

分 类 号：R96[医药卫生—药理学]